Zwiener C, Seeger S, Glauner T, Frimmel F H
Engler-Bunte-Institut, Water Chemistry, Universität Karlsruhe (TH), Germany.
Anal Bioanal Chem. 2002 Feb;372(4):569-75. doi: 10.1007/s00216-001-1210-x. Epub 2001 Dec 21.
The three metabolites hydroxyibuprofen (OH-Ibu), carboxyibuprofen (CA-Ibu), and carboxyhydratropic acid (CA-HA), also known from human metabolism of ibuprofen, could be identified in biodegradation experiments. Identification was based on EI mass spectra and comparison with literature data. Detection was performed by selective MS-MS measurements by GC-ion-trap MS and online methylation. Ibuprofen (Ibu), OH-Ibu, and CA-Ibu could be detected with a signal-to-noise ratio of 10:1 at a concentration of 2 nmol L(-1), CA-HA at 0.5 nmol L(-1). Degradation experiments in both biofilm reactors (BFR) and batch experiments with activated sludge (BAS) reveal OH-Ibu as the major metabolite under oxic conditions, and CA-HA under anoxic conditions. CA-Ibu was found under oxic and anoxic conditions almost only in the BAS. The metabolites together do not account for more than 10% of the initial concentration of Ibu.
在生物降解实验中可以鉴定出三种代谢产物，即羟基布洛芬（OH-Ibu）、羧基布洛芬（CA-Ibu）和羧基氢化阿托酸（CA-HA），这些在布洛芬的人体代谢中也有发现。鉴定基于电子轰击质谱（EI质谱）并与文献数据进行比较。检测通过气相色谱-离子阱质谱（GC-ion-trap MS）的选择性质谱-质谱（MS-MS）测量和在线甲基化进行。布洛芬（Ibu）、OH-Ibu和CA-Ibu在浓度为2 nmol L⁻¹时，信噪比为10:1时可被检测到，CA-HA在0.5 nmol L⁻¹时可被检测到。生物膜反应器（BFR）中的降解实验和活性污泥批次实验（BAS）表明，在有氧条件下主要代谢产物是OH-Ibu，在缺氧条件下是CA-HA。CA-Ibu几乎只在有氧和缺氧条件下的BAS中被发现。这些代谢产物加起来占Ibu初始浓度的比例不超过10%。
