Ozkan Sibel A, Uslu Bengi
Ankara University, Faculty of Pharmacy, Department of Analytical Chemistry, Turkey.
Anal Bioanal Chem. 2002 Feb;372(4):582-6. doi: 10.1007/s00216-001-1223-5. Epub 2002 Jan 12.
The oxidation of fluvastatin sodium on a glassy carbon electrode has been studied by use of a variety of voltammetric techniques. Different conditions were investigated to optimize the determination of fluvastatin sodium. The dependence of the intensities of currents and potentials on pH, concentration, scan rate, and nature of the buffer was investigated. Oxidation of fluvastatin sodium was found to be diffusion-controlled and irreversible. The best results for the determination of fluvastatin sodium were obtained by using differential pulse and square-wave voltammetric techniques in Britton-Robinson buffer at pH 10.04. Differential pulse and square-wave voltammetry at a glassy carbon electrode resulted in linear calibration in the range 8x10(-6) to 6x10(-4) mol L(-1) and detection limits of 1.07x10(-6) and 7.99x10(-7) mol L(-1), respectively. The proposed methods were successfully applied to the determination of the drug in capsules and biological fluids. Excipients did not interfere with the determination. Statistical validation revealed that the methods were free from significant systematic errors.
利用多种伏安技术研究了氟伐他汀钠在玻碳电极上的氧化情况。研究了不同条件以优化氟伐他汀钠的测定。考察了电流和电位强度对pH、浓度、扫描速率及缓冲液性质的依赖性。发现氟伐他汀钠的氧化是扩散控制且不可逆的。在pH 10.04的Britton-Robinson缓冲液中使用差分脉冲和方波伏安技术可获得测定氟伐他汀钠的最佳结果。在玻碳电极上进行差分脉冲和方波伏安法，在8×10⁻⁶至6×10⁻⁴ mol L⁻¹范围内得到线性校准，检测限分别为1.07×10⁻⁶和7.99×10⁻⁷ mol L⁻¹。所提出的方法成功应用于胶囊和生物流体中该药物的测定。辅料不干扰测定。统计验证表明这些方法无显著系统误差。
