(5Z)-4-溴-5-(溴亚甲基)-3-丁基-2(5H)-呋喃酮对枯草芽孢杆菌生物膜形成和群体运动的抑制作用
Inhibition of biofilm formation and swarming of Bacillus subtilis by (5Z)-4-bromo-5-(bromomethylene)-3-butyl-2(5H)-furanone.
作者信息
Ren D, Sims J J, Wood T K
机构信息
Department of Chemical Engineering and Molecular & Cellular Biology, University of Connecticut, Storrs 06269-3222, USA.
出版信息
Lett Appl Microbiol. 2002;34(4):293-9. doi: 10.1046/j.1472-765x.2002.01087.x.
AIMS
(5Z)-4-Bromo-5-(bromomethylene)-3-butyl-2(5H)-furanone(furanone) of the marine alga Delisea pulchra was synthesized, and its inhibition of swarming motility and biofilm formation of Bacillus subtilis was investigated.
METHODS AND RESULTS
Furanone was found to inhibit both the growth of B. subtilis and its swarming motility in a concentration-dependent way. In addition, as shown by confocal scanning laser microscopy, furanone inhibited the biofilm formation of B. subtilis. At 40 microg ml(-1), furanone decreased the biofilm thickness by 25%, decreased the number of water channels, and reduced the percentage of live cells by 63%.
CONCLUSIONS, SIGNIFICANCE AND IMPACT OF THE STUDY: Natural furanone has potential for controlling the multicellular behaviour of Gram-positive bacteria.
目的
合成了海洋藻类美丽德尔藻中的（5Z）-4-溴-5-（溴亚甲基）-3-丁基-2（5H）-呋喃酮（呋喃酮），并研究了其对枯草芽孢杆菌群体运动性和生物膜形成的抑制作用。
方法与结果
发现呋喃酮以浓度依赖的方式抑制枯草芽孢杆菌的生长及其群体运动性。此外，共聚焦扫描激光显微镜显示，呋喃酮抑制枯草芽孢杆菌生物膜的形成。在40微克/毫升时，呋喃酮使生物膜厚度降低25%，减少水通道数量，并使活细胞百分比降低63%。
研究的结论、意义及影响：天然呋喃酮具有控制革兰氏阳性菌多细胞行为的潜力。
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