通过复性动力学检测和定量金黄色葡萄球菌青霉素酶质粒脱氧核糖核酸
Detection and quantitation of Staphylococcus aureus penicillinase plasmid deoxyribonucleic acid by reassociation kinetics.
作者信息
Rush M, Novick R, DeLap R
出版信息
J Bacteriol. 1975 Dec;124(3):1417-23. doi: 10.1128/jb.124.3.1417-1423.1975.
Abstract
The quantity of penicillinase plasmid deoxyribonucleic acid (DNA) in various strains of Staphylococcus aureus has been determined by DNA-DNA reassociation kinetics. Specifically, 32P- or 125I-labeled denatured probes of purified plasmid DNA were reassociated in the presence of denatured DNAs isolated from the bacterial strains in question. The number of plasmid copies per cell was calculated from the effect of the latter nucleic acid samples on the reassociation rate of the radiolabeled probe. Among the S. aureus strains examined were monoplasmid, diplasmid and replication-defective representatives, and the effect of temperature on wild-type plasmid content was also investigated.
摘要
已通过DNA-DNA重缔合动力学测定了金黄色葡萄球菌各菌株中青霉素酶质粒脱氧核糖核酸（DNA）的量。具体而言，纯化质粒DNA的32P或125I标记的变性探针在从相关细菌菌株中分离出的变性DNA存在下进行重缔合。根据后一种核酸样品对放射性标记探针重缔合速率的影响计算每个细胞的质粒拷贝数。所检测的金黄色葡萄球菌菌株包括单质粒、双质粒和复制缺陷型代表菌株，还研究了温度对野生型质粒含量的影响。
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