Detection and quantitation of Staphylococcus aureus penicillinase plasmid deoxyribonucleic acid by reassociation kinetics.
作者信息
Rush M, Novick R, DeLap R
出版信息
J Bacteriol. 1975 Dec;124(3):1417-23. doi: 10.1128/jb.124.3.1417-1423.1975.
Abstract
The quantity of penicillinase plasmid deoxyribonucleic acid (DNA) in various strains of Staphylococcus aureus has been determined by DNA-DNA reassociation kinetics. Specifically, 32P- or 125I-labeled denatured probes of purified plasmid DNA were reassociated in the presence of denatured DNAs isolated from the bacterial strains in question. The number of plasmid copies per cell was calculated from the effect of the latter nucleic acid samples on the reassociation rate of the radiolabeled probe. Among the S. aureus strains examined were monoplasmid, diplasmid and replication-defective representatives, and the effect of temperature on wild-type plasmid content was also investigated.
摘要
相似文献
1
Detection and quantitation of Staphylococcus aureus penicillinase plasmid deoxyribonucleic acid by reassociation kinetics.
J Bacteriol. 1975 Dec;124(3):1417-23. doi: 10.1128/jb.124.3.1417-1423.1975.
2
Nucleic acid hybridization analysis of an integrated plasmid in Staphylococcus aureus.
J Bacteriol. 1975 Dec;124(3):1424-8. doi: 10.1128/jb.124.3.1424-1428.1975.
3
Genetic and molecular characterisation of resistance determinants in methicillin-resistant Staphylococcus-aureus.
J Med Microbiol. 1976 May;9(2):137-48. doi: 10.1099/00222615-9-2-137.
4
Studies on plasmid replication. II. In vivo transcription and its control in penicillinase plasmids from Staphylococcus aureus.
J Mol Biol. 1973 Sep 25;79(3):471-80. doi: 10.1016/0022-2836(73)90399-9.
5
Plasmid-specific transformation in Staphylococcus aureus.
J Bacteriol. 1973 Jul;115(1):139-45. doi: 10.1128/jb.115.1.139-145.1973.
6
Molecular characterization of two beta-lactamase-specifying plasmids isolated from Neisseria gonorrhoeae.
J Bacteriol. 1977 Aug;131(2):557-63. doi: 10.1128/jb.131.2.557-563.1977.
7
Plasmid-mediated beta-lactamase production in Neisseria gonorrhoeae.
Antimicrob Agents Chemother. 1977 Mar;11(3):528-33. doi: 10.1128/AAC.11.3.528.
8
Deoxyribonucleic acid reassociation in the classification of coagulase-positive staphylococci.
Arch Microbiol. 1978 May 30;117(2):183-8. doi: 10.1007/BF00402306.
9
NATURE AND INTERACTIONS OF THE GENETIC ELEMENTS GOVERNING PENICILLINASE SYNTHESIS IN STAPHYLOCOCCUS AUREUS.
J Bacteriol. 1965 Aug;90(2):467-80. doi: 10.1128/jb.90.2.467-480.1965.
10
Origin of the TEM-beta-lactamase gene found on plasmids.
J Bacteriol. 1975 Apr;122(1):250-6. doi: 10.1128/jb.122.1.250-256.1975.
引用本文的文献
1
Accessory gene regulator control of staphyloccoccal enterotoxin d gene expression.
J Bacteriol. 2004 Mar;186(6):1793-801. doi: 10.1128/JB.186.6.1793-1801.2004.
2
Coding sequence for the pT181 repC product: a plasmid-coded protein uniquely required for replication.
Proc Natl Acad Sci U S A. 1982 Jul;79(13):4108-12. doi: 10.1073/pnas.79.13.4108.
3
Control of plasmid R6K copy numbers in isogenic rep+ and rep Escherichia coli strains.
Mol Gen Genet. 1980 Feb;177(3):493-9. doi: 10.1007/BF00271489.
4
A simple and generally applicable procedure for releasing DNA from bacterial cells.
Appl Environ Microbiol. 1986 May;51(5):1138-40. doi: 10.1128/aem.51.5.1138-1140.1986.
5
Nucleic acid hybridization analysis of an integrated plasmid in Staphylococcus aureus.
J Bacteriol. 1975 Dec;124(3):1424-8. doi: 10.1128/jb.124.3.1424-1428.1975.
6
Mercury and organomercurial resistances determined by plasmids in Staphylococcus aureus.
J Bacteriol. 1977 Oct;132(1):197-208. doi: 10.1128/jb.132.1.197-208.1977.
本文引用的文献
1
POSSIBLE SYNTHESIS OF POLYRIBONUCLEOTIDES OF KNOWN BASE-TRIPLET SEQUENCES.
Nature. 1965 Apr 3;206:93. doi: 10.1038/206093b0.
2
DOMINANCE OF THE INDUCIBLE STATE IN STRAINS OF STAPHYLOCOCCUS AUREUS CONTAINING TWO DISTINCT PENICILLINASE PLASMIDS.
J Bacteriol. 1965 Aug;90(2):370-4. doi: 10.1128/jb.90.2.370-374.1965.
3
ANALYSIS BY TRANSDUCTION OF MUTATIONS AFFECTING PENICILLINASE FORMATION IN STAPHYLOCOCCUS AUREUS.
J Gen Microbiol. 1963 Oct;33:121-36. doi: 10.1099/00221287-33-1-121.
4
New type of restriction to the expression of a structural gene in bacteria.
Nature. 1967 Dec 23;216(5121):1191-2. doi: 10.1038/2161191a0.
5
Kinetics of renaturation of DNA.
J Mol Biol. 1968 Feb 14;31(3):349-70. doi: 10.1016/0022-2836(68)90414-2.
6
The problems of drug-resistant pathogenic bacteria. Extrachromosomal nature of drug resistance in Staphylococcus aureus.
Ann N Y Acad Sci. 1971 Jun 11;182:279-94. doi: 10.1111/j.1749-6632.1971.tb30664.x.
7
Penicillinase plasmid DNA from Staphylococcus aureus.
Proc Natl Acad Sci U S A. 1969 Aug;63(4):1304-10. doi: 10.1073/pnas.63.4.1304.
8
A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells.
Proc Natl Acad Sci U S A. 1967 May;57(5):1514-21. doi: 10.1073/pnas.57.5.1514.
9
Iodination of nucleic acids in vitro.
Biochemistry. 1971 May 25;10(11):1993-2000. doi: 10.1021/bi00787a005.
10
A variety of Staphylococcal plasmids present as multiple copies.
J Gen Microbiol. 1973 Dec;79(2):343-5. doi: 10.1099/00221287-79-2-343.
Zotero 插件