一种从细菌细胞中释放DNA的简单且普遍适用的方法。
A simple and generally applicable procedure for releasing DNA from bacterial cells.
作者信息
Heath L S, Sloan G L, Heath H E
出版信息
Appl Environ Microbiol. 1986 May;51(5):1138-40. doi: 10.1128/aem.51.5.1138-1140.1986.
Abstract
Treatment of Staphylococcus simulans biovar staphylolyticus cells with acetone before digestion with lysozyme made the cells susceptible to lysis by sodium dodecyl sulfate. This technique was found to be useful for releasing DNA from a wide variety of gram-positive and gram-negative organisms.
摘要
在用溶菌酶消化之前，先用丙酮处理模仿葡萄球菌溶菌生物变种细胞，可使这些细胞易于被十二烷基硫酸钠裂解。已发现该技术对于从多种革兰氏阳性和革兰氏阴性生物体中释放DNA很有用。
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