Otsuka H, Moskowitz M
J Cell Physiol. 1975 Oct;86(2 PT 2 SUPPL 1):379-87. doi: 10.1002/jcp.1040860410.
There is a low uptake of leucine in suspension cultures of 3T3 cells relative to the uptake in sparse monolayer cultures. The pattern of uptake of deoxyglucose is similar in suspension and monolayer cultures and changing the medium or adding serum stimulates uptake under both culture conditions. The uptake of uridine and adenosine is greater in suspension culture than in monolayer culture. Cells do not multiply in suspension culture but do multiply in monolayer culture and thus there is a correlation between uptake of leucine and conditions which stimulate cell multiplication, but not correlation of the uptake of deoxyglucose, uridine and adenosine with these conditions.
相对于稀疏单层培养中的摄取，3T3细胞悬浮培养中亮氨酸的摄取较低。悬浮培养和单层培养中脱氧葡萄糖的摄取模式相似，改变培养基或添加血清会在两种培养条件下刺激摄取。悬浮培养中尿苷和腺苷的摄取比单层培养中更高。细胞在悬浮培养中不增殖，但在单层培养中增殖，因此亮氨酸摄取与刺激细胞增殖的条件之间存在相关性，但脱氧葡萄糖、尿苷和腺苷的摄取与这些条件不存在相关性。
