Rekiki Abdessalem, Sidi-Boumedine Karim, Souriau Armel, Jemli Jemaa, Hammami Salah, Rodolakis Annie
Institut de la Recherche Vétérinaire de Tunisie, La Rabta.
Vet Res. 2002 Mar-Apr;33(2):215-22. doi: 10.1051/vetres:2002009.
Chlamydiosis is one of the major diseases that can lead to abortion in ewes. Since 1997, in 5 regions of Tunisia, Chlamydia-related abortions have been reported in 15 sheep and goat flocks. One hundred and sixty-six sera and 50 vaginal swab samples were collected from adult ewes. Chlamydial antigens were detected in 29 (58%) of the vaginal swabs using Enzyme Linked Immunosorbent Assay (ELISA) while 9 (18%) were positive by cell culture. Five strains were recovered from 4 different sheep flocks. Monoclonal antibody profiles and restriction fragment length polymorphism (RFLP) analysis of the 16S-23S rRNA spacer region showed that these isolates were C. abortus. Using amplified fragment length polymorphism (AFLP), these Tunisian strains were shown to exhibit the same pattern as strains isolated in France.
衣原体病是导致母羊流产的主要疾病之一。自1997年以来，在突尼斯的5个地区，15个绵羊和山羊群中报告了与衣原体相关的流产病例。从成年母羊中采集了166份血清和50份阴道拭子样本。使用酶联免疫吸附测定（ELISA）在29份（58%）阴道拭子中检测到衣原体抗原，而通过细胞培养有9份（18%）呈阳性。从4个不同的绵羊群中分离出5株菌株。对16S - 23S rRNA间隔区的单克隆抗体谱和限制性片段长度多态性（RFLP）分析表明，这些分离株为流产衣原体。使用扩增片段长度多态性（AFLP），这些突尼斯菌株显示出与在法国分离的菌株相同的模式。
