Souriau A, Rodolakis A
Vet Microbiol. 1986 Mar;11(3):251-9. doi: 10.1016/0378-1135(86)90027-1.
An enzyme linked immunosorbent assay (ELISA) for the detection of Chlamydia psittaci in vaginal swabs of aborted ewes and goats has been developed using microtiter plates coated with sheep anti-Chlamydia immunoglobulin G. This technique was compared to the direct isolation of the agent by plaque assay on McCoy cells. Among 89 specimens from animals in infected flocks, 58 were positive by both methods, seven were only positive by ELISA, and nine others were only positive by direct isolation (plaque assay). None of the 75 specimens from animals in healthy flocks gave a positive response in ELISA or the plaque assay. Unlike direct isolation in cell culture, the ELISA technique permitted the detection of Chlamydia even in the absence of special care in sampling and conservation of specimens.
已开发出一种酶联免疫吸附测定（ELISA）法，用于检测流产母羊和母山羊阴道拭子中的鹦鹉热衣原体，该方法使用包被有羊抗衣原体免疫球蛋白G的微量滴定板。将该技术与通过在 McCoy 细胞上进行蚀斑测定直接分离病原体的方法进行了比较。在来自受感染羊群动物的89份样本中，两种方法均呈阳性的有58份，仅ELISA呈阳性的有7份，仅直接分离（蚀斑测定）呈阳性的有9份。来自健康羊群动物的75份样本在ELISA或蚀斑测定中均未给出阳性反应。与细胞培养中的直接分离不同，ELISA技术即使在样本采集和保存时没有特别注意的情况下也能检测到衣原体。
