Teifke J P, Reimann I, Schirrmeier H
Institut für Infektionsmedizin, Bundesforschungsanstalt für Viruskrankheiten der Tiere (BFAV), Insel Riems, Germany.
J Comp Pathol. 2002 Feb-Apr;126(2-3):231-4. doi: 10.1053/jcpa.2001.0534.
Rabbit haemorrhagic disease (RHD) is usually peracute to acute, while subacute to chronic disease is rare. This paper describes gross and histopathological findings in four out of 20 rabbits aged 14 weeks, experimentally infected with one of two German field isolates of RHD virus. Eight rabbits survived the infection for 10 days and were killed after four of them, infected with 100 to 10 000 haemagglutination units, had started to develop progressive jaundice. Histopathologically, icteric livers showed severe subacute centrilobular bridging necrosis with calcification, and proliferation of periportal hepatocytes and bile ducts. Positive-strand RHDV RNA was detected by in-situ hybridization, mainly in periportal macrophages. Loss of the normal hepatic architecture, reparation (fibrosis) and hepatocellular regeneration, together with moderate inflammatory reaction, are signs of liver cirrhosis. These signs, observed in young rabbits given small doses of RHD virus, are interpreted as an unusual outcome of experimental inoculation.
兔出血性疾病（RHD）通常呈超急性至急性，而亚急性至慢性疾病较为罕见。本文描述了20只14周龄兔子中的4只经实验感染两种德国RHD病毒野外分离株之一后的大体和组织病理学发现。8只兔子在感染后存活了10天，其中4只感染了100至10000个血凝单位并开始出现进行性黄疸后被处死。组织病理学上，黄疸肝脏显示严重的亚急性小叶中央桥接坏死伴钙化，以及汇管区肝细胞和胆管增生。通过原位杂交检测到正链RHDV RNA，主要存在于汇管区巨噬细胞中。正常肝结构丧失、修复（纤维化）和肝细胞再生，以及中度炎症反应，是肝硬化的迹象。在给予小剂量RHD病毒的幼兔中观察到的这些迹象，被解释为实验接种的异常结果。
