Shien J H, Shieh H K, Lee L H
Department of Veterinary Medicine, National Chung Hsing University, Taichung, 403, Taiwan.
Res Vet Sci. 2000 Jun;68(3):255-9. doi: 10.1053/rvsc.1999.0372.
Adult and 4-5-week-old rabbits were inoculated subcutaneously with rabbit haemorrhagic disease virus (RHDV). Samples were prepared from various tissues at intervals postinoculation (PI) for the detection of viral RNA and antigens. Using a haemagglutination test (HAT), viral antigens were detected in the liver, bile and spleen of the adult rabbits at and after 36 h PI. The reverse transcription-polymerase chain reaction (RT - PCR) showed that RHDVRNA was present in the liver, bile and spleen as early as 18 hours PI, whereas lung, kidney, thymus, mesenteric lymph node and buffy coat were found to be positive after more than 26 hours PI. In addition, viral RNA in urine and faeces showed a variable positivity at and after 36 hours PI. In the young rabbits, RT - PCR showed that RHDVRNA was present as early as 1 day PI in the liver, bile, spleen and buffy coat; whereas lung, kidney, thymus, mesenteric lymph node and faeces were found to be positive at and after 2 days PI. Bile and spleen were the only samples in which viral RNA was detected throughout the length of the experiment. Virus was not reactivated in six recovered virus-inoculated rabbits treated with dexamethasone or a classical swine fever virus vaccine. Using a haemagglutination inhibition test and an ELISA, antibody titres increased rapidly from one week PI onwards, peaked at approximately three weeks of age, and were maintained throughout the length of the experiment.
将成年兔和4 - 5周龄的幼兔皮下接种兔出血性疾病病毒（RHDV）。在接种后（PI）的不同时间间隔从各种组织制备样本，用于检测病毒RNA和抗原。使用血凝试验（HAT），在接种后36小时及之后，在成年兔的肝脏、胆汁和脾脏中检测到病毒抗原。逆转录 - 聚合酶链反应（RT - PCR）显示，早在接种后18小时，肝脏、胆汁和脾脏中就存在RHDV RNA，而肺、肾、胸腺、肠系膜淋巴结和血沉棕黄层在接种后26小时以上才呈阳性。此外，尿液和粪便中的病毒RNA在接种后36小时及之后呈现出不同程度的阳性。在幼兔中，RT - PCR显示，早在接种后1天，肝脏、胆汁、脾脏和血沉棕黄层中就存在RHDV RNA；而肺、肾、胸腺、肠系膜淋巴结和粪便在接种后2天及之后呈阳性。胆汁和脾脏是在整个实验过程中均检测到病毒RNA的仅有的样本。在用地塞米松或经典猪瘟病毒疫苗处理的6只康复的接种病毒的兔子中，病毒未被重新激活。使用血凝抑制试验和ELISA，抗体滴度从接种后1周开始迅速升高，在大约3周龄时达到峰值，并在整个实验过程中保持。
