Kimura T, Mitsui I, Okada Y, Furuya T, Ochiai K, Umemura T, Itakura C
Laboratory of Comparative Pathology, Department of Veterinary Clinical Sciences, Hokkaido University, Sapporo 060-0818, Japan.
J Comp Pathol. 2001 Feb-Apr;124(2-3):134-41. doi: 10.1053/jcpa.2000.0440.
Adult rabbits were inoculated with liver homogenate from a rabbit that died in a Japanese outbreak of rabbit haemorrhagic disease (RHD). All experimentally infected rabbits died with typical clinical, gross and histological findings of RHD. Distribution of RHD virus in tissues of the infected rabbits was studied by non-isotopic in-situ hybridization. Both viral plus- and minus-strand RNAs were detected within the cytoplasm of hepatocytes, Kupffer cells and splenic and alveolar macrophages, mainly in morphologically intact cells. Strand-specific reverse transcriptase-polymerase chain reaction also demonstrated viral minus-strand RNA as well as plus-strand RNA in the liver, lung and spleen of infected rabbits. These results suggest that viral replication occurs not only in hepatocytes but also in macrophages. The infected macrophages may contribute to viral dissemination in RHD.
将成年兔子接种于一只在日本兔出血性疾病（RHD）暴发中死亡兔子的肝脏匀浆。所有经实验感染的兔子均死于具有RHD典型临床、大体和组织学表现的病症。通过非同位素原位杂交研究了RHD病毒在感染兔子组织中的分布。在肝细胞、库普弗细胞、脾巨噬细胞和肺泡巨噬细胞的细胞质内均检测到病毒正链和负链RNA，主要存在于形态完整的细胞中。链特异性逆转录聚合酶链反应也证实，在感染兔子的肝脏、肺和脾脏中存在病毒负链RNA以及正链RNA。这些结果表明，病毒复制不仅发生在肝细胞中，也发生在巨噬细胞中。受感染的巨噬细胞可能在RHD病毒传播中发挥作用。
