Tang Jie, Wang Zhili, Li Xin, Li Junlai, Shi Huaiyin
Department of Ultrasound, Chinese PLA General Hospital, Beijing, P R China.
Artif Cells Blood Substit Immobil Biotechnol. 2008;36(2):83-93. doi: 10.1080/10731190801932074.
To investigate the correlation between the expressions of human telomerase reverse transcriptase (hTERT) and vascular endothelial growth factor (VEGF) in prostate cancer (PCa) and benign prostatic hyperplasia (BPH), and to determine if hTERT was correlated with VEGF-promoted tumor cell proliferation in prostate cancer.
Immunohistochemistry was used to analyze the expressions of hTERT and VEGF in 60 cases of PCa and 60 cases of benign prostatic hyperplasia (BPH). Then their correlation in PCa was analyzed by Spearman correlative analysis.
The expressions of hTERT were detected in 38 cases of PCa and 10 cases of BPH. The expressions of VEGF were detected in 46 cases of PCa and 28 cases of BPH. The expressions of hTERT and VEGF in PCa were significantly higher than those in BPH (P < 0.05). As a result of correlation analysis, it was found that with an increase of the expression of VEGF, the expression of hTERT also increased in PCa. Significant correlation was observed between the expressions of hTERT and VEGF in PCa (r = 0.8333, P < 0.05). But there was no significant correlation between the expressions of hTERT and VEGF in BPH (r = 0.3156, P > 0.05).
All experiences above indicate that hTERT was one of the important proteins in the proliferation-promoting effect of VEGF on tumor cells in PCa.
探讨人端粒酶逆转录酶（hTERT）和血管内皮生长因子（VEGF）在前列腺癌（PCa）和良性前列腺增生（BPH）中的表达相关性，并确定hTERT是否与VEGF促进前列腺癌肿瘤细胞增殖相关。
采用免疫组织化学法分析60例PCa和60例良性前列腺增生（BPH）中hTERT和VEGF的表达。然后通过Spearman相关性分析其在PCa中的相关性。
在38例PCa和10例BPH中检测到hTERT的表达。在46例PCa和28例BPH中检测到VEGF的表达。PCa中hTERT和VEGF的表达明显高于BPH（P<0.05）。相关性分析结果发现，在PCa中随着VEGF表达的增加，hTERT的表达也增加。PCa中hTERT和VEGF的表达之间存在显著相关性（r=0.8333，P<0.05）。但BPH中hTERT和VEGF的表达之间无显著相关性（r=0.3156，P>0.05）。
上述所有实验表明，hTERT是VEGF促进PCa肿瘤细胞增殖作用中的重要蛋白之一。
