Nielsen F H, Myron D R, Givand S H, Zimmerman T J, Ollerich D A
J Nutr. 1975 Dec;105(12):1620-30. doi: 10.1093/jn/105.12.1620.
Nickel deficiency was produced in rats fed diet (containing 2-15 ng of mickel/g) based on dried skim mile, acid-washed ground corn, EDTA-extracted soy protein, and corn oil. Controls were fed a supplemental 3 mug of nickel/g of diet as NiCl2-6H2O. The rats were raised in plastic cages located inside laminar flow racks. Nickel deprivation resulted in several consistent pathological findings. These included: (1) increased perinatal mortality, (2) unthriftiness in young rats characterized by a rough coat and/or uneven hair development, (3) altered gross appearance (color) of the liver, (4) increased rate of alpha-glycerophosphate oxidation by liver homogenates, (5) decreased liver cholesterol, and (6) ultrastructural changes in the liver with the most obvious difference in the amount and organization of the rough endoplasmic reticulum. Nickel deficiency in rats tended to decrease growth, hematocrits, and liver total lipids and phospholipids.
给大鼠喂食以脱脂奶粉、酸洗玉米粉、乙二胺四乙酸提取的大豆蛋白和玉米油为基础的日粮（含镍量为2 - 15纳克/克），从而造成大鼠缺镍。对照组大鼠则喂食添加了3微克/克六水合氯化镍的日粮。大鼠饲养在层流架内的塑料笼中。缺镍导致了一些一致的病理结果。这些结果包括：（1）围产期死亡率增加；（2）幼鼠发育不良，表现为被毛粗糙和/或毛发发育不均；（3）肝脏外观（颜色）改变；（4）肝脏匀浆中α - 甘油磷酸氧化速率增加；（5）肝脏胆固醇含量降低；（6）肝脏超微结构改变，最明显的差异在于粗面内质网的数量和组织。大鼠缺镍往往会导致生长、血细胞比容以及肝脏总脂质和磷脂含量下降。
