Martino Elena, Franco Barbara, Piccoli Giovanni, Stocchi Vilberto, Perotto Silvia
Dipartimento di Biologia Vegetale dell'Università di Torino, Italy.
Mol Cell Biochem. 2002 Feb;231(1-2):179-85. doi: 10.1023/a:1014485420186.
A heavy metal tolerant strain of the ericoid mycorrhizal species Oidiodendron maius, isolated from soil heavily contaminated with zinc, was previously shown to tolerate high concentrations of zinc and cadmium ions in the growth medium. We have investigated some of the specific molecular responses of this fungal strain to the presence of increasing concentrations of zinc ions in the growth medium. In particular, we show that zinc ions induce a general change in the array of secreted proteins, with a shift towards the production of more basic, low molecular weight polypeptides. Some of these proteins were microsequenced and identified through homology search in databases. Among them are hydrolytic enzymes (nuclease, proteinase, lysozyme) and two superoxide dismutase isoforms. The latter are antioxidant enzymes known to play a role in heavy metal response in plants, animals and microorganisms.
从锌污染严重的土壤中分离出的一种耐重金属的欧石楠类菌根真菌——大隔孢伏革菌菌株，先前已证明其能耐受生长培养基中高浓度的锌离子和镉离子。我们研究了该真菌菌株对生长培养基中锌离子浓度增加的一些特定分子反应。特别地，我们发现锌离子会导致分泌蛋白阵列发生总体变化，向产生更多碱性、低分子量多肽的方向转变。对其中一些蛋白质进行了微量测序，并通过数据库中的同源性搜索进行了鉴定。其中包括水解酶（核酸酶、蛋白酶、溶菌酶）和两种超氧化物歧化酶同工型。后者是抗氧化酶，已知在植物、动物和微生物的重金属反应中发挥作用。
