LeClaire Ross D, Hunt Robert E, Bavari Sina
U.S. Army Medical Research Institute of Infectious Diseases, Frederick, Maryland 21702-5011, USA.
Infect Immun. 2002 May;70(5):2278-81. doi: 10.1128/IAI.70.5.2278-2281.2002.
We investigated the ability of two overlapping fragments of staphylococcal enterotoxin B (SEB), which encompass the whole toxin, to induce protection and also examined if passive transfer of chicken anti-SEB antibodies raised against the holotoxin could protect rhesus monkeys against aerosolized SEB. Although both fragments of SEB were highly immunogenic, the fragments failed to protect mice whether they were injected separately or injected together. Passive transfer of antibody generated in chickens (immunoglobulin Y [IgY]) against the whole toxin suppressed cytokine responses and was protective in mice. All rhesus monkeys treated with the IgY specific for SEB up to 4 h after challenge survived lethal SEB aerosol exposure. These findings suggest that large fragments of SEB may not be ideal for productive vaccination, but passive transfer of SEB-specific antibodies protects nonhuman primates against lethal aerosol challenge. Thus, antibodies raised in chickens against the holotoxin may have potential therapeutic value within a therapeutic window of opportunity after SEB encounter.
我们研究了葡萄球菌肠毒素B（SEB）的两个重叠片段（这两个片段涵盖了整个毒素）诱导保护作用的能力，还检测了用针对全毒素产生的鸡抗SEB抗体进行被动转移是否能保护恒河猴免受雾化SEB的侵害。尽管SEB的两个片段都具有高度免疫原性，但无论单独注射还是一起注射，这些片段都无法保护小鼠。鸡产生的针对全毒素的抗体（免疫球蛋白Y [IgY]）的被动转移可抑制细胞因子反应，并且对小鼠具有保护作用。在攻击后长达4小时用针对SEB的IgY治疗的所有恒河猴在致死性SEB气溶胶暴露中均存活。这些发现表明，SEB的大片段可能不是有效疫苗接种的理想选择，但SEB特异性抗体的被动转移可保护非人灵长类动物免受致死性气溶胶攻击。因此，鸡针对全毒素产生的抗体在接触SEB后的治疗机会窗内可能具有潜在的治疗价值。
