Bentes Gentil Arthur, Guimarães Juliana Rodrigues, Volotão Eduardo de Mello, Lanzarini Natália Maria, da Silva Alexandre Dos Santos, Gardinali Noemi Rovaris, Marchevsky Renato Sergio, Leite José Paulo Gagliardi, de Oliveira Jaqueline Mendes, Pinto Marcelo Alves
Laboratório de Desenvolvimento Tecnológico em Virologia, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro 21040-360, RJ, Brazil.
Laboratório de Virologia Comparada e Ambiental, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro 21040-360, RJ, Brazil.
Pharmaceutics. 2024 Aug 30;16(9):1149. doi: 10.3390/pharmaceutics16091149.
Immunoglobulins Y (IgY) purified from egg yolks of hens represents an attractive, cost-effective alternative for the development of new diagnostic and therapeutic platforms. In this study, we evaluated the therapeutic efficacy of rotavirus-specific IgY in a cynomolgus monkey () model. Animals were experimentally infected with human rotavirus Group A (RVA), the most common cause of severe acute diarrhoea among young children worldwide. Animals were administered human RVA (3.1 × 10 FFU/mL) by oral gavage, challenged with 2.5 mg of anti-RVA IgY orally, and monitored for five days according to clinical, haematological and biochemical parameters; serum electrolyte levels; viral shedding; and histopathological changes. Immunotherapy with anti-RVA IgY had a protective effect against severe rotavirus-induced enteritis in four of the ten treated monkeys, as evidenced by histopathological findings. Although only one animal had diarrhoea, all but one exhibited virus shedding regardless of the treatment.
从母鸡蛋黄中纯化的免疫球蛋白Y（IgY）是开发新型诊断和治疗平台的一种有吸引力且具成本效益的替代物。在本研究中，我们在食蟹猴模型中评估了轮状病毒特异性IgY的治疗效果。动物通过实验感染人A组轮状病毒（RVA），这是全球幼儿严重急性腹泻的最常见病因。通过口服灌胃给动物施用人类RVA（3.1×10 FFU/mL），口服给予2.5mg抗RVA IgY进行挑战，并根据临床、血液学和生化参数、血清电解质水平、病毒排泄以及组织病理学变化进行为期五天的监测。抗RVA IgY免疫疗法对十只接受治疗的猴子中的四只严重轮状病毒诱导的肠炎具有保护作用，组织病理学结果证明了这一点。尽管只有一只动物出现腹泻，但除一只外所有动物无论治疗与否均出现病毒排泄。
