Doukyu N, Nakano T, Okuyama Y, Aono R
Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
Appl Microbiol Biotechnol. 2002 Mar;58(4):543-6. doi: 10.1007/s00253-001-0919-y. Epub 2002 Jan 24.
Acinetobacter sp. strain ST-550 was isolated from fumus soil as an efficient indigo producer in the presence of organic solvent. The minimum inhibitory concentration of indole was 0.4 mg/ml for ST-550. ST-550 produced only a small amount of indigo (less than 0.01 microg/ml) when grown in the presence of indole at concentrations of 0.05 to 0.3 mg/ml without any organic solvent. However. ST-550 produced indigo effectively when grown in the presence of a large volume of diphenylmethane and a high level of indole: optimized conditions were 3 ml of a medium containing 0.3 ml diphenylmethane and 2.7 mg indole. Under these conditions, ST-550 produced 0.88 mg indigo (292 microg/mI medium).
不动杆菌属菌株ST - 550是从腐殖土中分离出来的，在有机溶剂存在的情况下是一种高效的靛蓝生产者。吲哚对ST - 550的最低抑菌浓度为0.4毫克/毫升。当ST - 550在不含任何有机溶剂、吲哚浓度为0.05至0.3毫克/毫升的条件下生长时，仅产生少量靛蓝（小于0.01微克/毫升）。然而，当在大量二苯甲烷和高浓度吲哚存在的情况下生长时，ST - 550能有效产生靛蓝：优化条件为3毫升含有0.3毫升二苯甲烷和2.7毫克吲哚的培养基。在这些条件下，ST - 550产生了0.88毫克靛蓝（292微克/毫升培养基）。
