新型不动杆菌 PP-2 菌株生长基质对靛蓝形成的影响及优化。
Influence and optimization of growth substrates on indigo formation by a novel isolate Acinetobacter sp. PP-2.
机构信息
Key Laboratory of Industrial Ecology and Environmental Engineering, MOE, School of Environmental and Biological Science and Technology, Dalian University of Technology, Dalian 116024, People's Republic of China.
出版信息
Bioresour Technol. 2010 Jun;101(12):4527-32. doi: 10.1016/j.biortech.2010.01.033. Epub 2010 Feb 13.
In this study, a novel indigo-producing bacterial strain PP-2 was isolated from activated sludge. It was identified as Acinetobacter sp. according to phylogenetic similarity of 16S rRNA gene sequence. This isolate was able to produce indigo from indole by utilizing a wide range of aromatic hydrocarbons. The results of SDS-PAGE analysis showed that the enzyme system induced by phenol was more abundant than that induced by other aromatic hydrocarbons. And the effects of metal ions on indigo production were also investigated, which indicated that the activity of cells induced with phenol could be inhibited by 0.5mM Fe(3+). Response surface methodology (RSM) was applied to optimize the process of indigo bio-production. The results exhibited that the maximal yield was achieved with 157.92 mg L(-1) phenol and 205.32 mg L(-1) indole. Under the optimal conditions, the indigo yield and transformation efficiency of indole were 202.92 mg L(-1) and 88%, respectively.
在这项研究中，我们从活性污泥中分离出一株新型靛蓝生产菌 PP-2。根据 16S rRNA 基因序列的系统发育相似性，将其鉴定为不动杆菌属。该分离株能够利用广泛的芳烃从吲哚中生产靛蓝。SDS-PAGE 分析结果表明，苯酚诱导的酶系统比其他芳烃诱导的酶系统更为丰富。此外，我们还研究了金属离子对靛蓝生产的影响，结果表明，苯酚诱导的细胞活性可以被 0.5mM Fe(3+)抑制。我们还应用响应面法（RSM）优化了靛蓝生物合成的过程。结果表明，在 157.92mg/L 苯酚和 205.32mg/L 吲哚的条件下，可获得最大产量。在最佳条件下，靛蓝的产量和吲哚的转化率分别为 202.92mg/L 和 88%。
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