Al-Khateeb S, Exterkate R A M, de Josselin de Jong E, Angmar-Månsson B, ten Cate J M
Department of Orthodontics, Jordan University of Science and Technology, Amman, Jordan.
Caries Res. 2002 Jan-Feb;36(1):25-30. doi: 10.1159/000057586.
Changes in the hydration state of enamel affect its optical qualities, such as light scattering and fluorescence. In this study, the rate of fluorescence loss was measured when incipient enamel lesions with different de-remineralization history were left to dehydrate. Four groups of lesions were studied. In groups A, B and C, the lesions were prepared in vitro in an acid-gel system. Group A was kept as control, and groups B and C were remineralized (4 weeks) without and with 1 ppm F in solution, respectively. Group D consisted of natural incipient lesions. Enamel fluorescence was measured for all lesions immediately after removal from water and subsequently at short intervals for 30 min. The change in fluorescence with dehydration varied between the groups. In lesions from groups A and B, it followed a double exponential decrease, while in lesions from groups C and D, it followed a mono-exponential decrease. In all groups, the fluorescence of sound surfaces declined mono-exponentially. The 'fractional fluorescence difference', defined as (L(sound) - L(carious) )/L(sound), became constant after periods of dehydration of about 5, 5, 20 and 5 min for groups A to D, respectively. The observation of the change of fluorescence with dehydration should be taken into consideration when planning studies that use fluorescence as an assessment method. However, it might also be used to gain insight into the properties for fluid transport inside the various lesions, relevant to de-remineralization or fluoride treatments.
牙釉质水合状态的变化会影响其光学性质，如光散射和荧光。在本研究中，测量了具有不同脱矿历史的早期牙釉质病变脱水时的荧光损失率。研究了四组病变。在A、B和C组中，病变在体外酸凝胶系统中制备。A组作为对照组，B组和C组分别在无氟和含1 ppm氟的溶液中再矿化4周。D组由天然早期病变组成。所有病变从水中取出后立即测量牙釉质荧光，随后每隔短时间测量30分钟。各组脱水时荧光的变化各不相同。A组和B组病变的荧光呈双指数下降，而C组和D组病变的荧光呈单指数下降。在所有组中，健康表面的荧光呈单指数下降。分别定义为（L（健康）-L（龋坏））/L（健康）的“荧光分数差异”在A至D组脱水约5、5、20和5分钟后变得恒定。在规划使用荧光作为评估方法的研究时，应考虑荧光随脱水变化的观察结果。然而，它也可用于深入了解各种病变内与脱矿或氟治疗相关的流体传输特性。
