Usatiuk P V, Tsvilikhovs'kyĭ M I, Aniiefiok A, Mel'nychuk D O
National Agrarian University, Ukraine, Kiev.
Fiziol Zh (1994). 2001;47(6):58-62.
The contains of blood plasma protein fractions and immunoglobulines were studied in adult cattle and in new born calves (just new born and age of 1, 2, 5 and 10 days) by the 10% PAAG EP (0.1% SDS) and radial immunodiffusion methods. It is shown that protein bands of 140-160 kD and immunoglobulines (G1, G2, M) are absent at the moment of birth. The physiology level of gamma-globulines and IgG1 reach at 1 day of life, while IgM--at second. After colostrum breeding of just new born calves the speed increasing of total plasma protein and gamma-globulines fraction are lowest (in 2.7 and 1.7 times respectively) in diarrhoea animals in compare to the health one. The maximum level of IgG1 concentration forms in disease animals only at 10 days of age. On the date obtained it is concluded the presence such new born calves which have low level of small intestine developing and disturbance of immunoglobulines absorption.
采用10%聚丙烯酰胺梯度电泳(0.1%十二烷基硫酸钠)和放射免疫扩散法,对成年牛以及新生犊牛(刚出生以及出生1、2、5和10天的犊牛)的血浆蛋白组分和免疫球蛋白进行了研究。结果显示,出生时不存在140 - 160 kD的蛋白条带以及免疫球蛋白(G1、G2、M)。γ-球蛋白和IgG1的生理水平在出生1天时达到,而IgM在出生2天时达到。与健康新生犊牛相比,新生腹泻犊牛初乳喂养后,血浆总蛋白和γ-球蛋白组分的增速最低(分别为2.7倍和1.7倍)。仅在患病动物出生10天时形成IgG1浓度的最高水平。根据所获数据得出结论,存在小肠发育水平低且免疫球蛋白吸收障碍的新生犊牛。