Naylor J M, Kronfeld D S, Bech-Nielsen S, Bartholomew R C
J Am Vet Med Assoc. 1977 Oct 1;171(7):635-8.
Plasma concentration of total protein (TP) was measured by refractometer in 76 calves, 1 to 8 days of age, on 3 farms. Three calves died before weaning at 5 weeks; all had plasma TP less than 6 g/dl. The frequency of disease before weaning was 17/29 (59%) in calves that had plasma TP of less than or equal to 6 g/dl, and this was significantly (P less than 0.001) less than the disease frequency of 9/47 (19%) in those with higher values of plasma TP. For 9 calves, plasma TP content was 4.72 +/- 0.22 (mean +/- SD) before ingestion of colostrum. The increment of plasma TP after colostrum feeding was 2.03 +/- 0.61 mg/dl (P less than 0.001). Plasma TP was as useful as serum zinc sulfate turbidity test results or total immunoglobins (as measured by radial immunodiffusion) in predicting disease susceptibility during the first 5 weeks of life, based on determinations made in 32 calves during the first 8 days of life. It was concluded that determination of plasma TP is the most convenient method of evaluating the immune status of calves under field conditions.
在3个农场中，用折射仪对76头1至8日龄的犊牛进行了总蛋白（TP）血浆浓度测定。3头犊牛在5周龄断奶前死亡；它们的血浆TP均低于6 g/dl。血浆TP小于或等于6 g/dl的犊牛断奶前的疾病发生率为17/29（59%），这显著低于（P<0.001）血浆TP值较高的犊牛的疾病发生率9/47（19%）。9头犊牛在摄入初乳前血浆TP含量为4.72±0.22（平均值±标准差）。初乳喂养后血浆TP的增量为2.03±0.61 mg/dl（P<0.0）。根据对32头犊牛出生后前8天的测定结果，血浆TP在预测犊牛出生后前5周的疾病易感性方面与血清硫酸锌浊度试验结果或总免疫球蛋白（通过放射免疫扩散测定）同样有用。得出的结论是，在野外条件下，测定血浆TP是评估犊牛免疫状态最便捷的方法。
