含糖类表面活性剂的三元混合脂质体对肺癌细胞生长的抑制作用显著增强。

Remarkably enhanced inhibitory effects of three-component hybrid liposomes including sugar surfactants on the growth of lung carcinoma cells.

作者信息

Ueoka Ryuichi, Matsumoto Yoko, Hirose Shigeru, Goto Koichi, Goto Masahiro, Furusaki Shintaro

机构信息

Graduate Course of Applied Chemistry, Sojo University, Ikeda, Kumamoto, Japan.

出版信息

Chem Pharm Bull (Tokyo). 2002 Apr;50(4):563-5. doi: 10.1248/cpb.50.563.

DOI:10.1248/cpb.50.563

PMID:11964013

Abstract

Sugar parts play important roles in recognizing molecules on the cell membranes. We successfully produced sugar-type micellar surfactants, lactonoalkylamide (LacCn), for the first time. Spherical vesicles, three-component hybrid liposomes, were obtained after the sonication of the mixture of L-alpha-dimyristoyl-phosphatidylcholine (DMPC), Tween 20 and LacCn (DMPC:Tween 20:LacCn=65:7:28). It is noteworthy that high inhibitory effects of the three-component hybrid liposomes composed of DMPC, Tween 20, and LacCn (DMPC:Tween 20:LacCn=65:7:28) on the growth of glioma (U251) and lung adenocarcinoma (RERF-LC-OK) cells were attained in vitro without any drug, although no significant inhibitory effects of any individual component (DMPC, Tween 20, LacCn) or the two-component hybrid liposomes of DMPC and Tween 20 on the growth of tumor cells examined were obtained.

摘要

糖类部分在识别细胞膜上的分子方面发挥着重要作用。我们首次成功制备了糖型胶束表面活性剂，即内酯烷基酰胺（LacCn）。将L-α-二肉豆蔻酰磷脂酰胆碱（DMPC）、吐温20和LacCn（DMPC:吐温20:LacCn = 65:7:28）的混合物超声处理后，得到了球形囊泡，即三组分混合脂质体。值得注意的是，由DMPC、吐温20和LacCn组成的三组分混合脂质体（DMPC:吐温20:LacCn = 65:7:28）在体外对胶质瘤（U251）和肺腺癌（RERF-LC-OK）细胞的生长具有高抑制作用，且无需任何药物，尽管所检测的任何单个组分（DMPC、吐温20、LacCn）或DMPC与吐温20的双组分混合脂质体对肿瘤细胞生长均未显示出显著抑制作用。