[杂交脂质体对杂交瘤细胞体外生长的特异性抑制作用]

[Specific inhibitory effect of hybrid liposomes on the growth of hybridoma cells in vitro].

作者信息

Imamura C, Kanno A, Mitsuoka C, Kitajima S, Inoue H, Iwahara M, Matsumoto Y, Ueoka R

机构信息

Graduate Course of Applied Chemistry, Kumamoto Institute of Technology, Japan.

出版信息

Yakugaku Zasshi. 1996 Dec;116(12):942-50. doi: 10.1248/yakushi1947.116.12_942.

DOI:10.1248/yakushi1947.116.12_942

PMID:8993232

Abstract

Remarkably high inhibitory effects of the hybrid liposomes composed of L-alpha-dimyristoyl-phosphatidylcholine (DMPC) and polyoxyethylenealkyl ether (C14(EO)n, n = 6-8 and C12(EO)n, n = 8-12)) on the growth of human lymphoma-human B-lymphocyte hybridoma (HF) cells in vitro were obtained. The hybrid liposomes composed of 90 mol% DMPC/10 mol% C14(EO)n (n = 6-8) or C12(EO)n (n = 8-12) were more fluid as compared with 90 mol% DMPC/10 mol% C14(EO)4 or C12(EO)n (n = 4, 23) hybrid liposomes on the basis of fluorescence polarization measurements. These results suggest that the inhibitory effects of the hybrid liposomes on the growth of HF cells should be related to the membrane fluidity. No toxicity to normal rats in vivo was observed in the experiment using 90 mol% DMPC/10 mol% C14(EO)7 or 90 mol% DMPC/10 mol% C12(EO)12 hybrid liposomes.

摘要

由L-α-二肉豆蔻酰磷脂酰胆碱（DMPC）和聚氧乙烯烷基醚（C14(EO)n，n = 6 - 8以及C12(EO)n，n = 8 - 12）组成的混合脂质体对人淋巴瘤 - 人B淋巴细胞杂交瘤（HF）细胞的体外生长具有显著的高抑制作用。基于荧光偏振测量，与90 mol% DMPC/10 mol% C14(EO)4或C12(EO)n（n = 4, 23）混合脂质体相比，由90 mol% DMPC/10 mol% C14(EO)n（n = 6 - 8）或C12(EO)n（n = 8 - 12）组成的混合脂质体流动性更强。这些结果表明，混合脂质体对HF细胞生长的抑制作用应与膜流动性有关。在使用90 mol% DMPC/10 mol% C14(EO)7或90 mol% DMPC/10 mol% C12(EO)12混合脂质体的实验中，未观察到对正常大鼠的体内毒性。