碱性磷酸酶自发插入并分配到模型脂筏中。
Spontaneous insertion and partitioning of alkaline phosphatase into model lipid rafts.
作者信息
Milhiet Pierre-Emmanuel, Giocondi Marie-Cécile, Baghdadi Omid, Ronzon Frédéric, Roux Bernard, Le Grimellec Christian
机构信息
Centre de Biochimie Structurale, CNRS UMR 5048 Université Montpellier I, INSERM UMR554, 29 rue de Navacelles, F-34090 Montpellier cedex, France.
出版信息
EMBO Rep. 2002 May;3(5):485-90. doi: 10.1093/embo-reports/kvf096. Epub 2002 Apr 18.
Abstract
Several cell surface eukaryotic proteins have a glycosylphosphatidylinositol (GPI) modification at the C-terminal end that serves as an anchor to the plasma membrane and could be responsible for the presence of GPI proteins in rafts, a type of functionally important membrane microdomain enriched in sphingolipids and cholesterol. In order to understand better how GPI proteins partition into rafts, the insertion of the GPI-anchored alkaline phosphatase (AP) was studied in real-time using atomic force microscopy. Supported phospholipid bilayers made of a mixture of sphingomyelin-dioleoylphosphatidylcholine containing cholesterol (Chl+) or not (Chl-) were used to mimic the fluid-ordered lipid phase separation in biological membranes. Spontaneous insertion of AP through its GPI anchor was observed inside both Chl+ and Chl- lipid ordered domains, but AP insertion was markedly increased by the presence of cholesterol.
摘要
几种细胞表面真核蛋白在其C末端有糖基磷脂酰肌醇（GPI）修饰，该修饰作为质膜的锚定物，可能是导致GPI蛋白存在于脂筏中的原因，脂筏是一种富含鞘脂和胆固醇的功能重要的膜微区。为了更好地理解GPI蛋白如何分配到脂筏中，使用原子力显微镜实时研究了GPI锚定碱性磷酸酶（AP）的插入。由含有胆固醇（Chl+）或不含胆固醇（Chl-）的鞘磷脂 - 二油酰磷脂酰胆碱混合物制成的支持磷脂双层用于模拟生物膜中的液 - 序脂质相分离。在Chl+和Chl-脂质有序域内均观察到AP通过其GPI锚自发插入，但胆固醇的存在显著增加了AP的插入。
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