Kalaĭdzhieva S, Dimitrova-Dikanarova D, Stanislavov R, Nakov L
Akush Ginekol (Sofiia). 1999;38(1):52-6.
The aim of the present investigation was to establish the frequency of sperm antibodies in patients with etiologically unexplained infertility, and to compare the demonstrated frequencies with the results from y control group of y healthy blood donors, as well as with the results of other investigators. The gelatin agglutination test of Kibrick and the tray agglutination test of Friberg were applied to test 244 sera from infertile patients and 50 sera from healthy blood donors at the Laboratory of Immunology of Reproduction, Department of Biology, Medical University of Sofia. For the infertile patients, relevant sperm antibody titers were demonstrated in 2.5% (titer > or = 16) for the Kibrick method, and in 7% (titer > or = 32) for the Fiberg method. The test of Kibrick did not reveal significant antibody titers in the healthy controls, while the test of Friberg showed sperm antibodies in 2% of the blood donors. Fisher's exact test demonstrated extremely significant correlation (p < 0.0001) between the presence of sperm antibodies in sers of patients with unexplained infertility as revealed by the tests of Kibrick and Friberg. Most often mixed agglutinates were demonstrated in the Friberg test. In contrast with the results of other investigators head-to-head agglutinins were observed more often in male sera, while tail-to-tail agglutinins--in female sera. Finally, the results from the present investigation, as well as the analyzed literature data showed a low frequency of anti-sperm immunity in the Bulgarian population. The established high degree of correlation between the tests of Kibrick and Friberg, the good reproducibility of the results and the low cost of these methods confirm their appropriate use for the diagnosis of sperm antibodies in patients with unexplained infertility.
本研究的目的是确定病因不明的不孕症患者中精子抗体的出现频率，并将所证实的频率与健康献血者对照组的结果以及其他研究者的结果进行比较。在索非亚医科大学生物学系生殖免疫学实验室，采用基布里克明胶凝集试验和弗里伯格平板凝集试验，对244份不育患者血清和50份健康献血者血清进行检测。对于不育患者，基布里克法显示相关精子抗体滴度在2.5%（滴度≥16），弗里伯格法显示在7%（滴度≥32）。基布里克试验在健康对照中未发现显著抗体滴度，而弗里伯格试验在2%的献血者中显示有精子抗体。费舍尔精确检验表明，基布里克试验和弗里伯格试验所揭示的不明原因不孕症患者血清中精子抗体的存在之间存在极其显著的相关性（p<0.0001）。弗里伯格试验中最常出现混合凝集物。与其他研究者的结果相反，在男性血清中头对头凝集素更常见，而在女性血清中尾对尾凝集素更常见。最后，本研究结果以及分析的文献数据表明，保加利亚人群中抗精子免疫的频率较低。基布里克试验和弗里伯格试验之间所确立的高度相关性、结果的良好可重复性以及这些方法的低成本，证实了它们适用于诊断不明原因不孕症患者的精子抗体。
