Alenina Natalia, Baranova Tatjana, Smirnow Eugene, Bader Michael, Lippoldt Andrea, Patkin Eugene, Walther Thomas
Department of Cardiology, University Hospital Benjamin Franklin, Berlin-Buch, Germany.
J Histochem Cytochem. 2002 May;50(5):691-6. doi: 10.1177/002215540205000510.
The Mas proto-oncogene encodes a G-protein-coupled receptor with the common seven transmembrane domains and may be involved in the actions of angiotensins. Because Mas is highly expressed in testis, we investigated the cell type-specificity and the onset of expression of the gene in this organ. Using an RNase protection assay, it could be shown that neither whole testes nor cultured Sertoli and Leydig cells of 12-day-old mice express Mas mRNA. Mas expression is first detected in 18-day-old mice and thereafter increases continuously until 6 months of age. By in situ hybridization, the expression could be localized to Leydig cells and Sertoli cells, the signals being much more pronounced in the former. A weak signal was detected in primary spermatocytes. The strong ontogenetically controlled and cell type-specific expression of this membrane-bound receptor in testis implicates a role for the Mas proto-oncogene in testis maturation and function.
Mas原癌基因编码一种具有常见七跨膜结构域的G蛋白偶联受体，可能参与血管紧张素的作用。由于Mas在睾丸中高度表达，我们研究了该基因在该器官中的细胞类型特异性和表达起始情况。使用核糖核酸酶保护试验可以表明，12日龄小鼠的整个睾丸以及培养的支持细胞和间质细胞均不表达Mas mRNA。Mas表达首先在18日龄小鼠中检测到，此后持续增加直至6月龄。通过原位杂交，表达可定位于间质细胞和支持细胞，前者的信号更为明显。在初级精母细胞中检测到微弱信号。这种膜结合受体在睾丸中强烈的个体发育控制和细胞类型特异性表达暗示Mas原癌基因在睾丸成熟和功能中起作用。
