Hong Hyo-Bong, Chang Yoon-Seok, Nam In-Hyun, Fortnagel Peter, Schmidt Stefan
School of Environmental Science and Engineering, Pohang University of Science and Technology, Pohang 790-784, Korea.
Appl Environ Microbiol. 2002 May;68(5):2584-8. doi: 10.1128/AEM.68.5.2584-2588.2002.
Aerobic biotransformation of the diaryl ethers 2,7-dichlorodibenzo-p-dioxin and 1,2,3,4-tetrachlorodibenzo-p-dioxin by the dibenzo-p-dioxin-utilizing strain Sphingomonas wittichii RW1, producing corresponding metabolites, was demonstrated for the first time. Our strain transformed 2,7-dichlorodibenzo-p-dioxin, yielding 4-chlorocatechol, and 1,2,3,4-tetrachlorodibenzo-p-dioxin, producing 3,4,5,6-tetrachlorocatechol and 2-methoxy-3,4,5,6-tetrachlorophenol; all of these compounds were unequivocally identified by mass spectrometry both before and after N,O-bis(trimethylsilyl)-trifluoroacetamide derivatization by comparison with authentic standards. Additional experiments showed that strain RW1 formed a second metabolite, 2-methoxy-3,4,5,6-tetrachlorophenol, from the original degradation product, 3,4,5,6-tetrachlorocatechol, by methylation of one of the two hydroxy substituents.
利用二苯并对二噁英的鞘氨醇单胞菌RW1菌株对二芳基醚2,7-二氯二苯并对二噁英和1,2,3,4-四氯二苯并对二噁英进行需氧生物转化，产生相应代谢产物，这是首次得到证实。我们的菌株转化了2,7-二氯二苯并对二噁英，生成4-氯邻苯二酚，以及1,2,3,4-四氯二苯并对二噁英，生成3,4,5,6-四氯邻苯二酚和2-甲氧基-3,4,5,6-四氯苯酚；通过与标准品比较，在N,O-双(三甲基硅基)-三氟乙酰胺衍生化前后，所有这些化合物均通过质谱法明确鉴定。额外的实验表明，RW1菌株通过将两个羟基取代基之一甲基化，从原始降解产物3,4,5,6-四氯邻苯二酚形成了第二种代谢产物2-甲氧基-3,4,5,6-四氯苯酚。
