Yu Y-M, Chang W-C, Chang C-T, Hsieh C-L, Tsai C E
Department of Nutrition, China Medical College, Taichung, Taiwan.
Diabetes Metab. 2002 Apr;28(2):107-14.
The effects of supplementation of young barley leaf extract (BL) and/or antioxidative vitamins C and E on different low-density lipoprotein (LDL) subfractions susceptibility to oxidation and free radical scavenging activities in patients with type 2 diabetes were evaluated.
Thirty-six type 2 diabetic patients were enrolled in this study. The subjects received one of the following supplements daily for 4 weeks: 15 g BL, 200 mg vitamin C and 200 mg vitamin E (CE), or BL plus CE (BL + CE).
The lucigenin-chemiluminescence (CL) and luminol-CL levels in blood were significantly reduced in all groups. Vitamin E content of LDL subfractions increased significantly following supplements, especially for BL + CE group. The percent increase of lag times in the BL + CE was significantly higher than those in the BL or CE group. The antioxidative effect of BL + CE was the greatest for small, dense LDL (Sd-LDL) with further increases in percentage of lag times 4 folds compared to BL alone.
Our results indicate that supplementation with BL may help to scavenge oxygen free radicals, save the LDL-vitamin E content, and inhibit LDL oxidation. Furthermore, the addition of vitamins C and E to BL can inhibit the Sd-LDL oxidation more effectively, which may protect against vascular diseases in type 2 diabetic patients.
评估补充嫩大麦叶提取物（BL）和/或抗氧化维生素C和E对2型糖尿病患者不同低密度脂蛋白（LDL）亚组分氧化易感性及自由基清除活性的影响。
36例2型糖尿病患者纳入本研究。受试者每天接受以下补充剂之一，持续4周：15克BL、200毫克维生素C和200毫克维生素E（CE），或BL加CE（BL + CE）。
所有组血液中的光泽精化学发光（CL）和鲁米诺-CL水平均显著降低。补充后LDL亚组分的维生素E含量显著增加，尤其是BL + CE组。BL + CE组的延迟时间增加百分比显著高于BL或CE组。BL + CE对小而密LDL（Sd-LDL）的抗氧化作用最大，与单独使用BL相比，延迟时间百分比进一步增加了4倍。
我们的结果表明，补充BL可能有助于清除氧自由基、保存LDL-维生素E含量并抑制LDL氧化。此外，在BL中添加维生素C和E可以更有效地抑制Sd-LDL氧化，这可能预防2型糖尿病患者的血管疾病。
