Strässer Katja, Masuda Seiji, Mason Paul, Pfannstiel Jens, Oppizzi Marisa, Rodriguez-Navarro Susana, Rondón Ana G, Aguilera Andres, Struhl Kevin, Reed Robin, Hurt Ed
BZH, University of Heidelberg, Im Neuenheimer Feld 328, D-69120 Heidelberg, Germany.
Nature. 2002 May 16;417(6886):304-8. doi: 10.1038/nature746. Epub 2002 Apr 28.
The essential yeast proteins Yra1 and Sub2 are messenger RNA export factors that have conserved counterparts in metazoans, designated Aly and UAP56, respectively. These factors couple the machineries that function in splicing and export of mRNA. Here we show that both Yra1 and Sub2 are stoichiometrically associated with the heterotetrameric THO complex, which functions in transcription in yeast. We also show that Sub2 and Yra1 interact genetically with all four components of the THO complex (Tho2, Hpr1, Mft1 and Thp2). Moreover, these components operate in the export of bulk poly(A)(+) RNA as well as of mRNA derived from intronless genes. Both Aly and UAP56 associate with human counterparts of the THO complex. Together, these data define a conserved complex, designated the TREX ('transcription/export') complex. The TREX complex is specifically recruited to activated genes during transcription and travels the entire length of the gene with RNA polymerase II. Our data indicate that the TREX complex has a conserved role in coupling transcription to mRNA export.
酵母必需蛋白Yra1和Sub2是信使核糖核酸（mRNA）输出因子，在后生动物中有保守的对应物，分别称为Aly和UAP56。这些因子将参与mRNA剪接和输出的机制联系起来。我们在此表明，Yra1和Sub2都与异源四聚体THO复合物化学计量相关，THO复合物在酵母转录中发挥作用。我们还表明，Sub2和Yra1与THO复合物的所有四个组分（Tho2、Hpr1、Mft1和Thp2）存在遗传相互作用。此外，这些组分在整体多聚腺苷酸（poly(A)(+)）RNA以及来自无内含子基因的mRNA的输出中发挥作用。Aly和UAP56都与THO复合物的人类对应物相关联。这些数据共同定义了一个保守的复合物，称为TREX（“转录/输出”）复合物。TREX复合物在转录过程中被特异性招募到激活的基因上，并与RNA聚合酶II一起沿着基因全长移动。我们的数据表明，TREX复合物在将转录与mRNA输出联系起来方面具有保守作用。
