Candan Ferda, Gültekin Füsun, Candan Ferhan
University of Cumhuriyet, Faculty of Science and Art, Department of Chemistry, Sivas, Turkey.
Cell Biochem Funct. 2002 Jun;20(2):95-8. doi: 10.1002/cbf.947.
Peroxidation of the membrane lipid structure of red blood cell leads to haemolysis and anaemia in haemodialysis patients. Dietary constituents of antioxidant vitamins and trace elements may play an important role in protecting against oxidant damage. In this study, the effects of supplementation of vitamin C and zinc on osmotic fragility and lipid peroxidation of erythrocytes were investigated in 34 zinc-deficient haemodialysis patients. Sixteen sex- and age-matched normal volunteers acted as controls. Patients were randomized to receive vitamin C (250 mg day(-1)), zinc (20 mg day(-1)) or a placebo treatment for 3 months. The levels of vitamin C, zinc, malondialdehyde (MDA) and osmotic fragility were measured initially and 3 months after supplementation. Mean serum concentration of vitamin C and zinc increased significantly in the groups at the end of the respective study periods. Supplementation with vitamin C and zinc improved osmotic fragility, and decreased the level of MDA in the groups, but some side-effects (i.e. nausea, vomiting, fever, muscle pain, weakness) were observed during the zinc treatment. The results showed that the supplementation of both treatments decreased osmotic fragilty and MDA in zinc-deficient haemodialysis patients. However, vitamin C treatment was found to be safer than zinc supplementation.
红细胞膜脂质结构的过氧化会导致血液透析患者发生溶血和贫血。抗氧化维生素和微量元素的饮食成分可能在预防氧化损伤方面发挥重要作用。在本研究中，对34例锌缺乏的血液透析患者补充维生素C和锌对红细胞渗透脆性和脂质过氧化的影响进行了研究。16名年龄和性别匹配的正常志愿者作为对照。患者被随机分为接受维生素C（250毫克/天）、锌（20毫克/天）或安慰剂治疗3个月。在补充前和补充3个月后测量维生素C、锌、丙二醛（MDA）水平和渗透脆性。在各自研究期结束时，各治疗组的维生素C和锌平均血清浓度显著升高。补充维生素C和锌改善了各治疗组的渗透脆性并降低了MDA水平，但在锌治疗期间观察到一些副作用（如恶心、呕吐、发热、肌肉疼痛、虚弱）。结果表明，两种治疗均降低了锌缺乏血液透析患者的渗透脆性和MDA水平。然而，发现维生素C治疗比补充锌更安全。
