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使用固相C1q和富含蛋白A的金黄色葡萄球菌作为指示系统检测IgG聚集体或免疫复合物。

Detection of IgG aggregates or immune complexes using solid-phase C1q and protein A-rich Staphylococcus aureus as an indicator system.

作者信息

Farrell C, Sogaard H, Svehag S E

出版信息

Scand J Immunol. 1975;4(7):673-80. doi: 10.1111/j.1365-3083.1975.tb02675.x.

DOI:10.1111/j.1365-3083.1975.tb02675.x
PMID:1198069
Abstract

A radioimmunoassay for detection of C1q-binding IgG aggregates and antigen-IgG antibody complexes is described. The assay makes use of solid-phase C1q and 32p-labelled protein A-rich Staphylococcus aureus as an indicator system. Both 19S and heavier IgG aggregates that fixed C1q were detected. The sensitivity of the assay permitted detection of heavy (19-25S) IgG aggregates at a concentration of 8 mug/ml or less. The results indicated that detection of IgG in this assay is dependent on the degree of IgG polymerization and the molar ratio between the solid-phase C1q and the IgG polymers. Albumin-anti-albumin complexes, preformed at equilibrium with antibody to antigen molar ratios of 2:1 to 3:1 and at antigen concentrations of 25 to 40 mug/ml, were also detectable using the described radioimmunoassay.

摘要

本文描述了一种用于检测C1q结合IgG聚集体和抗原-IgG抗体复合物的放射免疫分析方法。该分析方法利用固相C1q和32P标记的富含蛋白A的金黄色葡萄球菌作为指示系统。检测到了固定C1q的19S及更重的IgG聚集体。该分析方法的灵敏度能够检测浓度为8微克/毫升或更低的重（19-25S）IgG聚集体。结果表明，该分析方法中IgG的检测取决于IgG聚合程度以及固相C1q与IgG聚合物之间的摩尔比。使用所描述的放射免疫分析方法，还可检测在抗体与抗原摩尔比为2:1至3:1且抗原浓度为25至40微克/毫升的平衡条件下预先形成的白蛋白-抗白蛋白复合物。

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引用本文的文献

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Prevalence and persistence of C1q binding activity in healthy subjects.健康受试者中C1q结合活性的患病率和持续性
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Studies on circulating soluble immune complexes of the liver disease. 6. Comparative studies of 125I-pRF inhibition assay, 125I-Clq inhibition assay and 125I-Clq binding assay.
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