Nielsen H, Svehag S R
Acta Pathol Microbiol Scand C. 1976 Aug;84(4):261-9. doi: 10.1111/j.1699-0463.1976.tb00029.x.
The applicability of a complement consumption assay as a means by which to detect IgG aggregates and immune complexes in serum was examined. Both heavy (greater than or equal to 19S) and intermediate (11-17S) IgG aggregates were detected and the sensitivity of the assay was greater than or equal to 10 mug aggregated IgG/ml. BSA anti-BSA complexes, formed in slight antibody excess, were detected at a BSA concentration of 200 ng/ml. NHS stored at 4degreesC for greater than or equal to 2-3 weeks or at -20degreesC for more than 3 months developed distinct anticomplementarity (AC). This background AC, due to IgG aggregate formation, was reduced by heating the serum at 56degreesC for 50 min prior to testing. A similar reduction of AC and C1q fixation was observed when IgG aggregated at 61degreesC or 63degreesC was heated further at 56degreesC for 50 min. The abatement of AC could not be correlated to a change in IgG aggregation size. In contrast, AC of preformed antigen-antibody complexes was not reduced by this heat treatment.
研究了补体消耗试验作为检测血清中IgG聚集体和免疫复合物的一种方法的适用性。检测到重链（大于或等于19S）和中链（11 - 17S）IgG聚集体，该试验的灵敏度大于或等于10μg聚集IgG/ml。在抗体略过量时形成的牛血清白蛋白（BSA）-抗BSA复合物，在BSA浓度为200 ng/ml时被检测到。在4℃储存大于或等于2 - 3周或在-20℃储存超过3个月的新鲜正常血清（NHS）产生明显的抗补体性（AC）。这种由于IgG聚集体形成导致的背景AC，通过在检测前将血清在56℃加热50分钟而降低。当在61℃或63℃聚集的IgG在56℃进一步加热50分钟时，观察到AC和C1q固定有类似程度的降低。AC的减轻与IgG聚集大小的变化无关。相反，预先形成的抗原 - 抗体复合物的AC不会因这种热处理而降低。
