用于检测人血清中免疫复合物、IgG聚集体和细菌产物的C1q偏离试验。
C1q deviation test for the detection of immune complexes, aggregates of IgG, and bacterial products in human serum.
作者信息
Sobel A T, Bokisch V A, Müller-Eberhard H J
出版信息
J Exp Med. 1975 Jul 1;142(1):139-50. doi: 10.1084/jem.142.1.139.
Abstract
This report describes a new, rapid, sensitive, and quantitative method for the detection of immune complexes, endotoxins, and other complement activating materials in patients sera utilizing the ability of these substances to react with isolated C1q. The procedure is based on the inhibition of radiolabeled C1q binding to sensitized sheep erythrocytes by C1q-reactive substances in pathological sera. The C1q deviation test may be performed on 50 mu1 of serum, using 1 mug of radiolabeled C1q per sample. The procedure may be completed in 1.5-2 h, it is capable of detecting 5 mug of aggregated human IgG per ml of serum, and its coefficient of variation is 4.2%. Application of the test to the study of 193 sera from 43 patients with Dengue hemorrhagic fever showed a positive correlation between degree of C1q deviation and severity of disease.
摘要
本报告描述了一种新的、快速、灵敏且定量的方法，用于检测患者血清中的免疫复合物、内毒素及其他补体激活物质，该方法利用这些物质与分离的C1q发生反应的能力。该程序基于病理血清中C1q反应性物质对放射性标记的C1q与致敏绵羊红细胞结合的抑制作用。C1q偏差试验可在50μl血清上进行，每个样品使用1μg放射性标记的C1q。该程序可在1.5 - 2小时内完成，能够检测每毫升血清中5μg聚集的人IgG，其变异系数为4.2%。将该试验应用于对43例登革出血热患者的193份血清的研究，结果显示C1q偏差程度与疾病严重程度呈正相关。
相似文献
1
C1q deviation test for the detection of immune complexes, aggregates of IgG, and bacterial products in human serum.用于检测人血清中免疫复合物、IgG聚集体和细菌产物的C1q偏离试验。
J Exp Med. 1975 Jul 1;142(1):139-50. doi: 10.1084/jem.142.1.139.
2
Circulating immune complexes in the serum in systemic lupus erythematosus and in carriers of hepatitis B antigen. Quantitation by binding to radiolabeled C1q.系统性红斑狼疮患者及乙肝抗原携带者血清中的循环免疫复合物。通过与放射性标记的C1q结合进行定量分析。
J Clin Invest. 1974 Aug;54(2):297-309. doi: 10.1172/JCI107765.
3
4
Detection of IgG aggregates or immune complexes using solid-phase C1q and protein A-rich Staphylococcus aureus as an indicator system.使用固相C1q和富含蛋白A的金黄色葡萄球菌作为指示系统检测IgG聚集体或免疫复合物。
Scand J Immunol. 1975;4(7):673-80. doi: 10.1111/j.1365-3083.1975.tb02675.x.
5
Circulating immune complexes in Lyme arthritis. Detection by the 125I-C1q binding, C1q solid phase, and Raji cell assays.莱姆关节炎中的循环免疫复合物。通过¹²⁵I-C1q结合法、C1q固相法和拉吉细胞试验进行检测。
J Clin Invest. 1979 Mar;63(3):468-77. doi: 10.1172/JCI109324.
6
Solid-phase Clq-binding fluorescence immunoassay for detection of circulating immune complexes.用于检测循环免疫复合物的固相Clq结合荧光免疫测定法。
J Clin Microbiol. 1982 Mar;15(3):456-64. doi: 10.1128/jcm.15.3.456-464.1982.
7
Detection of immune complexes. The use of radioimmunoassays with Clq and monoclonal rheumatoid factor.免疫复合物的检测。使用含补体C1q的放射免疫测定法和单克隆类风湿因子。
J Clin Invest. 1977 May;59(5):990-1001. doi: 10.1172/JCI108722.
8
Critical aspects of the 125I-C1q binding assay for detecting immune complexes in tumor patients.用于检测肿瘤患者免疫复合物的125I-C1q结合试验的关键方面。
J Clin Lab Immunol. 1984 Jun;14(2):87-92.
9
Dynamics of interaction between complement-fixing antibody/dsDNA immune complexes and erythrocytes. In vitro studies and potential general applications to clinical immune complex testing.补体结合抗体/dsDNA免疫复合物与红细胞之间相互作用的动力学。体外研究及在临床免疫复合物检测中的潜在广泛应用。
J Clin Invest. 1985 Jan;75(1):102-11. doi: 10.1172/JCI111660.
引用本文的文献
1
Identification of Leishmania donovani antigen in circulating immune complexes of visceral leishmaniasis subjects for diagnosis.检测内脏利什曼病患者循环免疫复合物中的杜氏利什曼原虫抗原用于诊断。
PLoS One. 2017 Aug 18;12(8):e0182474. doi: 10.1371/journal.pone.0182474. eCollection 2017.
2
Role of CD61+ cells in thrombocytopenia of dengue patients.CD61+ 细胞在登革热患者血小板减少症中的作用。
Int J Hematol. 2012 Nov;96(5):600-10. doi: 10.1007/s12185-012-1175-x. Epub 2012 Sep 18.
3
Alternative complement pathway deregulation is correlated with dengue severity.替代补体途径失调与登革热的严重程度相关。
PLoS One. 2009 Aug 26;4(8):e6782. doi: 10.1371/journal.pone.0006782.
4
Manipulation of cell surface macromolecules by flaviviruses.黄病毒对细胞表面大分子的操控。
Adv Virus Res. 2003;59:229-74. doi: 10.1016/s0065-3527(03)59007-8.
5
Studies on circulating immune complexes of the liver diseases. 5. Anti-DNA antibodies and C1q binding activity.肝脏疾病循环免疫复合物的研究。5. 抗DNA抗体与C1q结合活性
Gastroenterol Jpn. 1980 Apr;15(2):167-172. doi: 10.1007/BF02774932.
6
Circulating immune complexes in patients with gram negative septic shock.革兰氏阴性败血症休克患者的循环免疫复合物
Intensive Care Med. 1980;6(2):123-7. doi: 10.1007/BF01683357.
7
[Detection of complement-binding immune complexes by means of 51Cr-labeled indicator cells--a new radioimmunoassay (author's transl)].用51Cr标记指示细胞检测补体结合免疫复合物——一种新的放射免疫测定法（作者译）
Klin Wochenschr. 1980 May 2;58(9):479-82. doi: 10.1007/BF01476803.
8
Circulating immune complexes in dermatologic disease.皮肤病中的循环免疫复合物。
Springer Semin Immunopathol. 1981;4(3):221-40. doi: 10.1007/BF01892179.
9
The effect of fibrinopeptides A and B on experimental allergic encephalomyelitis.纤维蛋白肽A和B对实验性变应性脑脊髓炎的作用。
Clin Exp Immunol. 1980 Apr;40(1):49-59.
10
Circulating immune complexes in Plasmodium knowlesi infected Kra, and merozoite vaccinated Rhesus monkeys.诺氏疟原虫感染的克拉猴和裂殖子疫苗接种的恒河猴体内的循环免疫复合物。
Clin Exp Immunol. 1982 May;48(2):315-20.
本文引用的文献
1
Two types of gamma-globulin differing in carbohydrate content.两种碳水化合物含量不同的γ球蛋白。
Proc Soc Exp Biol Med. 1956 Oct;93(1):146-50. doi: 10.3181/00379727-93-22690.
2
3
4
Circulating immune complexes in the serum in systemic lupus erythematosus and in carriers of hepatitis B antigen. Quantitation by binding to radiolabeled C1q.系统性红斑狼疮患者及乙肝抗原携带者血清中的循环免疫复合物。通过与放射性标记的C1q结合进行定量分析。
J Clin Invest. 1974 Aug;54(2):297-309. doi: 10.1172/JCI107765.
5
The potential pathogenic role of complement in dengue hemorrhagic shock syndrome.补体在登革出血热休克综合征中的潜在致病作用。
N Engl J Med. 1973 Nov 8;289(19):996-1000. doi: 10.1056/NEJM197311082891902.
6
Effect of chemical and enzymatic radioiodination on in vitro human Clq activities.化学和酶促放射性碘化对体外人补体C1q活性的影响。
J Immunol. 1973 Mar;110(3):820-8.
7
C1q protein of human complement.人类补体C1q蛋白
Biochemistry. 1972 Aug 29;11(18):3443-50. doi: 10.1021/bi00768a018.
8
Circulating immune complexes in inflammatory bowel disease.炎症性肠病中的循环免疫复合物
Clin Exp Immunol. 1973 Jun;14(2):219-26.
9
A method of trace iodination of proteins for immunologic studies.一种用于免疫学研究的蛋白质微量碘化方法。
Int Arch Allergy Appl Immunol. 1966;29(2):185-9. doi: 10.1159/000229699.
10
Binding of soluble immune complexes to human lymphoblastoid cells. II. Use of Raji cells to detect circulating immune complexes in animal and human sera.可溶性免疫复合物与人淋巴母细胞的结合。II. 利用拉吉细胞检测动物和人血清中的循环免疫复合物。
J Exp Med. 1974 Nov 1;140(5):1230-44. doi: 10.1084/jem.140.5.1230.
Zotero 插件