多位点序列分型与脉冲场凝胶电泳用于鉴定从环境和临床来源分离的单核细胞增生李斯特菌菌株的比较分析
Comparative analysis of multilocus sequence typing and pulsed-field gel electrophoresis for characterizing Listeria monocytogenes strains isolated from environmental and clinical sources.
作者信息
Revazishvili Tamara, Kotetishvili Mamuka, Stine O Colin, Kreger Arnold S, Morris J Glenn, Sulakvelidze Alexander
机构信息
Department of Epidemiology and Preventive Medicine, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA.
出版信息
J Clin Microbiol. 2004 Jan;42(1):276-85. doi: 10.1128/JCM.42.1.276-285.2004.
Abstract
One hundred seventy-five Listeria monocytogenes strains were characterized by serotyping, pulsed-field gel electrophoresis (PFGE), and multilocus sequence typing (MLST) based on loci in actA, betL, hlyA, gyrB, pgm, and recA. One hundred twenty-two sequence types (STs) were identified by MLST based on allelic profiles of the four housekeeping genes (betL, gyrB, pgm, and recA), and 34 and 38 alleles were identified for hlyA and actA, respectively. Several actA and hlyA alleles appeared to be predominantly associated with clinical isolates. MLST differentiated most of the L. monocytogenes strains better than did PFGE, and the discriminating ability of PFGE was better than that of serotyping. Several strains with different serotypes were found, by MLST and PFGE, to have very closely related genetic backgrounds, which suggested possible "antigen switching" among them. MLST can be a useful typing tool for differentiating L. monocytogenes strains (including strains undistinguishable by PFGE typing and serotyping), and it may be of value during investigations of food-borne outbreaks of listeriosis.
摘要
基于actA、betL、hlyA、gyrB、pgm和recA基因座，通过血清分型、脉冲场凝胶电泳（PFGE）和多位点序列分型（MLST）对175株单核细胞增生李斯特菌进行了特征分析。基于四个管家基因（betL、gyrB、pgm和recA）的等位基因谱，通过MLST鉴定出122种序列类型（STs），hlyA和actA分别鉴定出34个和38个等位基因。几种actA和hlyA等位基因似乎主要与临床分离株相关。与PFGE相比，MLST对大多数单核细胞增生李斯特菌菌株的区分能力更强，PFGE的鉴别能力优于血清分型。通过MLST和PFGE发现，几种不同血清型的菌株具有非常密切相关的遗传背景，这表明它们之间可能存在“抗原转换”。MLST可以作为区分单核细胞增生李斯特菌菌株（包括PFGE分型和血清分型无法区分的菌株）的有用分型工具，在食源性李斯特菌病暴发调查中可能具有重要价值。
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