Stevenson Karen, Hughes Valerie M, de Juan Lucía, Inglis Neil F, Wright Frank, Sharp J Michael
Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik EH26 0PZ, Scotland, United Kingdom.
J Clin Microbiol. 2002 May;40(5):1798-804. doi: 10.1128/JCM.40.5.1798-1804.2002.
Five pigmented isolates of Mycobacterium avium subsp. paratuberculosis were examined by pulsed-field gel electrophoresis (PFGE), IS900 restriction fragment length polymorphism (IS900-RFLP), and IS1311 polymorphism analysis using PCR. All of the pigmented isolates exhibited one of three distinct PFGE profiles with SnaBI, designated 9, 10, and 11, and with SpeI, designated 7, 8, and 9, which generated three multiplex profiles designated [9-7], [10-8], and [11-9]. All of the pigmented isolates had the same IS900-RFLP BstEII and PvuII profiles. The IS900-RFLP BstEII profile was new, but the IS900-RFLP PvuII profile corresponded to PvuII type 6 of a sheep strain described by Cousins and colleagues (D. V. Cousins, S. N. Williams, A. Hope, and G. J. Eamens, Aust. Vet. J. 78:184-190, 2000). IS1311-PCR analysis typed all of the pigmented isolates as sheep (S) strains. The genetic relationship between pigmented and nonpigmented isolates was investigated by using multiplex PFGE data from the analysis of both the 5 pigmented isolates and 88 nonpigmented isolates of M. avium subsp. paratuberculosis from a variety of host species and geographic locations. It was possible to classify the isolates into two distinct types designated type I, comprising the pigmented isolates, and type II, comprising the nonpigmented isolates, which exhibit a very broad host range.
采用脉冲场凝胶电泳（PFGE）、IS900限制性片段长度多态性分析（IS900-RFLP）以及利用PCR进行的IS1311多态性分析，对5株鸟分枝杆菌副结核亚种色素沉着分离株进行了检测。所有色素沉着分离株在用SnaBI酶切时呈现出三种不同PFGE图谱中的一种，分别命名为9、10和11，在用SpeI酶切时呈现出另外三种不同PFGE图谱中的一种，分别命名为7、8和9，由此产生了三种复合图谱，分别命名为[9-7]、[10-8]和[11-9]。所有色素沉着分离株具有相同的IS900-RFLP BstEII和PvuII图谱。IS900-RFLP BstEII图谱是新发现的，但IS900-RFLP PvuII图谱与Cousins及其同事描述的绵羊菌株的PvuII 6型相对应（D. V. Cousins、S. N. Williams、A. Hope和G. J. Eamens，《澳大利亚兽医杂志》78:184 - 190，2000年）。IS1311-PCR分析将所有色素沉着分离株鉴定为绵羊（S）型菌株。利用来自对5株色素沉着分离株和88株来自多种宿主物种和地理位置的鸟分枝杆菌副结核亚种非色素沉着分离株进行分析的复合PFGE数据，研究了色素沉着分离株和非色素沉着分离株之间的遗传关系。有可能将这些分离株分为两种不同类型，命名为I型，包括色素沉着分离株，和II型，包括非色素沉着分离株，后者具有非常广泛的宿主范围。
