丝孢酵母属物种核糖体DNA基因间隔区1区域的序列分析
Sequence analysis of the ribosomal DNA intergenic spacer 1 regions of Trichosporon species.
作者信息
Sugita Takashi, Nakajima Masamitsu, Ikeda Reiko, Matsushima Toshiharu, Shinoda Takako
机构信息
Department of Microbiology, Meiji Pharmaceutical University, Kiyose, Tokyo 204-8588, Japan.
出版信息
J Clin Microbiol. 2002 May;40(5):1826-30. doi: 10.1128/JCM.40.5.1826-1830.2002.
Abstract
We determined the sequence of the intergenic spacer (IGS) 1 region, which is located between the 26S and 5S rRNA genes, in 25 species of the genus TRICHOSPORON: IGS 1 sequences varied in length from 195 to 719 bp. Comparative sequence analysis suggested that the divergence of IGS 1 sequences has been greater than that of the internal transcribed spacer regions. We also identified five genotypes of T. asahii, which is a major causative agent of deep-seated trichosporonosis, based on the IGS 1 sequences of 43 strains. Most of the isolates that originated in Japan were of genotype 1, whereas the American isolates were of genotype 3 or 5. Our results suggest that analysis of IGS regions provides a powerful method to distinguish between phylogenetically closely related species and that a geographic substructure may exist among T. asahii clinical isolates.
摘要
我们测定了25种丝孢酵母属（TRICHOSPORON）物种中位于26S和5S rRNA基因之间的基因间隔区（IGS）1区域的序列：IGS 1序列长度在195至719 bp之间变化。比较序列分析表明，IGS 1序列的分歧大于内部转录间隔区。我们还基于43株菌株的IGS 1序列鉴定出了阿萨希丝孢酵母（T. asahii）的五种基因型，阿萨希丝孢酵母是深部丝孢酵母病的主要病原体。大多数源自日本的分离株属于基因型1，而美国的分离株属于基因型3或5。我们的结果表明，IGS区域分析提供了一种区分系统发育关系密切的物种的有力方法，并且阿萨希丝孢酵母临床分离株之间可能存在地理亚结构。
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