Sugita T, Nishikawa A, Shinoda T
Department of Microbiology, Meiji College of Pharmacy, Tanashi, Tokyo, Japan.
J Clin Microbiol. 1998 Sep;36(9):2742-4. doi: 10.1128/JCM.36.9.2742-2744.1998.
Trichosporon asahii is a major causative agent of deep-seated trichosporonosis, which has a high mortality rate. To detect T. asahii, we have developed specific oligonucleotide primers based on the internal transcribed spacer regions of this organism's genome. Amplification products were selectively obtained from only T. asahii DNA; the DNAs of other Trichosporon species, as well as those of medically relevant yeasts such as Candida albicans, Cryptococcus neoformans, and Malassezia furfur, were not amplified. This detection system will be useful as a microbiological tool for the diagnosis of trichosporonosis.
阿萨希毛孢子菌是深部毛孢子菌病的主要病原体,该病死亡率很高。为了检测阿萨希毛孢子菌,我们基于该生物体基因组的内部转录间隔区开发了特异性寡核苷酸引物。仅从阿萨希毛孢子菌DNA中选择性地获得扩增产物;其他毛孢子菌属物种的DNA,以及医学上相关的酵母如白色念珠菌、新型隐球菌和糠秕马拉色菌的DNA均未被扩增。该检测系统作为诊断毛孢子菌病的微生物学工具将很有用。