Neale Matthew J, Ramachandran Madhu, Trelles-Sticken Edgar, Scherthan Harry, Goldman Alastair S H
Department of Molecular Biology and Biotechnology, The University of Sheffield, Sheffield S10 2TN, United Kingdom.
Mol Cell. 2002 Apr;9(4):835-46. doi: 10.1016/s1097-2765(02)00498-7.
We have studied the repair of a DNA-DSB created by the VMA1-derived endonuclease in mutants that have different levels of Spo11-DSBs: WT (sae2), few (hop1), and none (spo11-Y135F). In spo11-Y135F and hop1 cells, intrachromosomal repair is more frequent than in WT and sae2 cells. In spo11-Y135F cells there was no chromosome pairing or synapsis and a faster turnover of resected DNA. Compared to WT and sae2 cells, spo11-Y135F and hop1 cells have a greater proportion of long resection tracts. The data suggest that high levels of Spo11-DSBs are required for normal regulation of resection, even at a DSB created by another protein. WT control over resection could be important for directing repair to be interchromosomal, increasing the chance of creating interhomolog connections essential to meiotic segregation.
我们研究了在具有不同水平Spo11-DSB的突变体中，由VMA1衍生的核酸内切酶产生的DNA双链断裂（DNA-DSB）的修复情况：野生型（sae2）、少量（hop1）和无（spo11-Y135F）。在spo11-Y135F和hop1细胞中，染色体内修复比野生型和sae2细胞更频繁。在spo11-Y135F细胞中，没有染色体配对或联会，且切除的DNA周转更快。与野生型和sae2细胞相比，spo11-Y135F和hop1细胞中长切除片段的比例更高。数据表明，即使在由另一种蛋白质产生的DSB处，正常的切除调节也需要高水平的Spo11-DSB。野生型对切除的控制对于引导修复为染色体间修复可能很重要，增加了产生对减数分裂分离至关重要的同源连接的机会。
