Hiroi M, Stanczyk F Z, Goebelsmann U, Brenner P F, Lumkin M E, Mishell D R
Steroids. 1975 Sep;26(3):373-86. doi: 10.1016/0039-128x(75)90082-3.
A radioimmunoassay (RIA) method for measuring medroxyprogesterone acetate (MPA, Provera) in serum has been developed utilizing benzene:iso-octane extraction, 3H-MPA to assess procedural losses, goat anti-MPA-3-(0-carboxymethyl) oxime-bovine serum albumin serum and dextran-coated charcoal separation. Control serum blanks were undetectable, 200 pg/ml of MPA was measurable with a high reliability, and intra- and interassay coefficients of variation were 6 and 13 percent, respectively. MPA added to control serum was quantitatively recovered. Serum MPA levels measured in 2 women after ingestion of 10 mg MPA rose to 3.4 to 4.4 ng/ml within 1 to 4 hours after oral intake and fell rapidly thereafter to 0.3 to 0.6 ng/ml within 24 hours. Insertion of Silastic intra-vaginal rings (IVRs), containing 100 or 200 mg of MPA, into 4 women for periods of 3 weeks resulted in a rapid rise of serum MPA after insertion, rather stable MPA levels of 0.9 to 1.6 ng/ml while the IVRs were in place, and a rapid decline of serum MPA following IVR removal. Serum estradiol-17beta and progesterone concentrations, measured about 3 times a week in these patients, indicated that ovulation was consistently inhibited. The serum MPA levels observed in this study were approximately 5 times lower than those reported by other investigators using a double-antibody RIA of MPA in unextracted serum.
已开发出一种用于测量血清中醋酸甲羟孕酮（MPA，安宫黄体酮）的放射免疫分析（RIA）方法，该方法利用苯：异辛烷萃取、³H-MPA评估操作损失、山羊抗MPA-3-(O-羧甲基)肟-牛血清白蛋白血清以及葡聚糖包被活性炭分离。对照血清空白检测不到，200 pg/ml的MPA可高度可靠地测量，批内和批间变异系数分别为6%和13%。添加到对照血清中的MPA可定量回收。两名女性摄入10 mg MPA后，血清MPA水平在口服后1至4小时内升至3.4至4.4 ng/ml，此后在24小时内迅速降至0.3至0.6 ng/ml。将含有100或200 mg MPA的硅橡胶阴道环（IVR）插入4名女性体内3周，插入后血清MPA迅速升高，IVR在位期间MPA水平相当稳定，为0.9至1.6 ng/ml，IVR取出后血清MPA迅速下降。在这些患者中每周约测量3次血清雌二醇-17β和孕酮浓度，结果表明排卵持续受到抑制。本研究中观察到的血清MPA水平比其他研究者在未提取血清中使用MPA双抗体RIA报告的水平低约5倍。
