污染的红细胞对OKT3介导的外周血单个核细胞多克隆激活的影响。
Effect of contaminating red blood cells on OKT3-mediated polyclonal activation of peripheral blood mononuclear cells.
作者信息
Song Samuel, Goodwin Joseph, Zhang Jenny, Babbitt Bruce, Lathey Janet L
机构信息
Research Department, Cellcor Inc., Newton, Massachusetts 02139, USA.
出版信息
Clin Diagn Lab Immunol. 2002 May;9(3):708-12. doi: 10.1128/cdli.9.3.708-712.2002.
Erythrocytes are typically present as impurities in the majority of peripheral blood mononuclear cell (PBMC) preparations. This study was undertaken to investigate the effects of contaminating red blood cells (RBC) on the ability of OKT3 to activate CD4(+) and CD8(+) T cells. Surprisingly, the levels of gamma interferon, tumor necrosis factor alpha, and interleukin-1 beta (IL-1 beta) produced by PBMC upon stimulation by OKT3 were increased (P < 0.05) in a dose-dependent manner when increasing amounts of autologous RBC (RBC-to-PBMC ratios of 2:1, 10:1, and 50:1) were spiked into PBMC preparations. The OKT3-driven induction of the IL-2 receptor (CD25) and the proliferation of T lymphocytes in response to phorbol myristate acetate were not affected by the addition of RBC.
在大多数外周血单个核细胞（PBMC）制剂中，红细胞通常作为杂质存在。本研究旨在探讨污染的红细胞（RBC）对OKT3激活CD4(+)和CD8(+) T细胞能力的影响。令人惊讶的是，当向PBMC制剂中加入越来越多的自体红细胞（红细胞与PBMC的比例为2:1、10:1和50:1）时，OKT3刺激PBMC产生的γ干扰素、肿瘤坏死因子α和白细胞介素-1β（IL-1β）水平呈剂量依赖性增加（P < 0.05）。添加红细胞并不影响OKT3驱动的IL-2受体（CD25）的诱导以及T淋巴细胞对佛波醇肉豆蔻酸酯乙酸盐的增殖反应。
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