桔青霉（NCIM 1146）混合功能氧化酶系统的研究。
Study of mixed function oxidase system in Aspergillus ochraceus (NCIM 1146).
机构信息
Department of Biochemistry, Shivaji University, Kolhapur, 416 004 India.
出版信息
Indian J Microbiol. 2007 Dec;47(4):304-9. doi: 10.1007/s12088-007-0056-0. Epub 2008 Jan 11.
Aspergillus ochraceus (NCIM-1146) has shown the ability to degrade cholesterol, camphor and naphthalene, when 96 h grown mycelium incubated in medium containing these organic compounds. Presence of higher level of electron transport components and biotransformation enzyme activity were observed in Aspergillus ochraceus, when grown in potato dextrose medium for 96 h. The enzyme activity preferred NADPH as a cofactor and shows inhibition in the presence of CO, indicating cytochrome P-450 mediated reactions. A significant increase in the levels of electron transport components and biotransformation enzyme activity were observed in presence of different inducers (viz. cholesterol, camphor, naphthalene, veratrole, phenobarbital, n-hexane, kerosene and saffola oil) when compared with mycelium incubated in same way with similar conditions for 2 min incubation. Analyses of the products of cholesterol and camphor using HPLC and GCMS confirm the degradation of these compounds.
土曲霉（NCIM-1146）在含有这些有机化合物的培养基中培养 96 小时后，其菌丝体显示出降解胆固醇、樟脑和萘的能力。当在马铃薯葡萄糖培养基中培养 96 小时时，观察到土曲霉中存在更高水平的电子传递成分和生物转化酶活性。该酶活性优先使用 NADPH 作为辅助因子，并在 CO 存在下显示抑制作用，表明其为细胞色素 P-450 介导的反应。与以相同方式在相同条件下孵育 2 分钟的菌丝体相比，当存在不同诱导剂（即胆固醇、樟脑、萘、藜芦醇、苯巴比妥、正己烷、煤油和红花油）时，观察到电子传递成分和生物转化酶活性水平显著增加。使用 HPLC 和 GCMS 对胆固醇和樟脑的产物进行分析，证实了这些化合物的降解。
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