印度尼西亚与肠胃炎相关的诺如病毒特征分析。

Characterization of Norwalk-like virus associated with gastroenteritis in Indonesia.

作者信息

Subekti D S, Tjaniadi P, Lesmana M, Simanjuntak C, Komalarini S, Digdowirogo H, Setiawan B, Corwin A L, Campbell J R, Porter K R, Oyofo B A

机构信息

U.S. Naval Medical Research Unit No. 2, Jakarta, Indonesia.

出版信息

J Med Virol. 2002 Jun;67(2):253-8. doi: 10.1002/jmv.2215.

DOI:10.1002/jmv.2215

PMID:11992587

Abstract

Norwalk Virus and Norwalk-like viruses (NLVs) are reportedly responsible for 2.5-4.0% of nonbacterial acute gastroenteritis (NBAG) worldwide. To help clarify the impact of NLVs on NBAG in Indonesia, stool specimens from 102 patients, 74 with NBAG and 28 with BAG, were screened for the presence of NLVs, using a reverse transcription-polymerase chain reaction (RT-PCR) assay. The specimens were subtyped using prototype-specific oligonucleotide probes and were sequenced and compared with published NLV sequences. Of the 102 specimens examined, 31 (30%) were found to be positive for NLVs. Type-specific probe analysis of the RT-PCR products indicated that 31 isolates hybridized to UK1 (Taunton agent) and UK3/4 (Hawaii agent/Snow Mountain agent) prototype strains. The results of this study indicate that prototype strains of NV or NLVs co-circulate in Indonesia and contribute to the overall level of acute gastroenteritis throughout the region.

摘要

据报道，诺如病毒和诺如样病毒（NLVs）在全球非细菌性急性肠胃炎（NBAG）病例中占2.5%-4.0%。为了明确NLVs对印度尼西亚NBAG的影响，采用逆转录聚合酶链反应（RT-PCR）分析法，对102例患者的粪便样本进行检测，其中74例患有NBAG，28例患有细菌性肠胃炎（BAG），以筛查NLVs的存在情况。使用原型特异性寡核苷酸探针对样本进行亚型分型，并进行测序，然后与已发表的NLV序列进行比较。在检测的102份样本中，有31份（30%）被发现NLVs呈阳性。RT-PCR产物的型特异性探针分析表明，31株分离株与UK1（汤顿毒株）和UK3/4（夏威夷毒株/雪山毒株）原型毒株杂交。本研究结果表明，NV或NLVs的原型毒株在印度尼西亚共同流行，并导致了该地区急性肠胃炎的总体发病水平。