Senior Alan E, Nadanaciva Sashi, Weber Joachim
Department of Biochemistry and Biophysics, University of Rochester Medical Center, Box 712, 601 Elmwood Avenue, Rochester, NY 14642, USA.
Biochim Biophys Acta. 2002 Feb 15;1553(3):188-211. doi: 10.1016/s0005-2728(02)00185-8.
ATP synthesis by oxidative phosphorylation and photophosphorylation, catalyzed by F1F0-ATP synthase, is the fundamental means of cell energy production. Earlier mutagenesis studies had gone some way to describing the mechanism. More recently, several X-ray structures at atomic resolution have pictured the catalytic sites, and real-time video recordings of subunit rotation have left no doubt of the nature of energy coupling between the transmembrane proton gradient and the catalytic sites in this extraordinary molecular motor. Nonetheless, the molecular events that are required to accomplish the chemical synthesis of ATP remain undefined. In this review we summarize current state of knowledge and present a hypothesis for the molecular mechanism of ATP synthesis.
由F1F0 - ATP合酶催化的氧化磷酸化和光合磷酸化合成ATP，是细胞产生能量的基本方式。早期的诱变研究在一定程度上描述了其机制。最近，几个原子分辨率的X射线结构描绘了催化位点，亚基旋转的实时视频记录也让人们对这个非凡分子马达中跨膜质子梯度与催化位点之间的能量耦合性质不再怀疑。尽管如此，完成ATP化学合成所需的分子事件仍不明确。在这篇综述中，我们总结了当前的知识状态，并提出了一个关于ATP合成分子机制的假说。
