Matsubasa Tadashi, Uchino Takako, Karashima Shinnyo, Kondo Yuichi, Maruyama Kenichi, Tanimura Masako, Endo Fumio
Ashikita Institution for Developmental Disabilities, Kumamoto, Japan.
Free Radic Res. 2002 Feb;36(2):189-93. doi: 10.1080/10715760290006510.
Very low birth weight (VLBW) infants can be subjected to oxidative stress in the course of intensive care. We measured 8-hydroxydeoxyguanosine (8-OHdG), a biomarker of oxidative stress, and estimated the degree of oxidative stress in such infants. We also examined if the administered oxygen was related to oxidative stress. Urine samples of 50 Japanese VLBW infants [birth weights: 956.3+/-277.6g, and gestational ages: 28.0+/-2.6 weeks (mean +/- SD)] were collected on various postnatal days and 8-OHdG levels were determined using an ELISA kit. Sixteen term infants served as normal controls. As body weights at sampling increased, the average levels of urinary 8-OHdG decreased. 8-Hydroxydeoxyguanosine levels were: infants under 1000g, 29.5+/-16.4 micromol/mol creatinine (n = 24); 1000-1500g, 23.8+/-14.9 (n = 12); over 1500g, 16.1+/-8.5 (n = 14); and control, 10.9+/-7.2 (n = 16). Significant differences were found between <1000g group and > or = 1500g group (p = 0.0030), <1000g group and control (p < 0.0001), and 1000-1500g group and control (p = 0.0108). Also as postconceptional age at sampling increased, the average levels of 8-OHdG decreased. 8-Hydroxydeoxyguanosine levels were: infants before 252 days (36 weeks) of postconception: 27.4+/-15.5 micromol/mol creatinine (n = 34); after 252 days, 18.2+/-12.5 (n = 16). Differences between <252 days group and control (p < 0.0001), and <252 days group and > or = 252 days groups (p = 0.0253) were statistically significant. Among the three groups based on ambient oxygen concentration (21%, 22-29%, and > or = 30%) there was no significant difference (p = 0.417). The more premature the infants were, the more intense was the oxidative stress, hence, it is the prematurity rather than the administered oxygen which causes oxidative stress in VLBW infants. Drury et al. ["Urinary 8-hydroxydeoxyguanosine in infants and children" Free Radic. Res. 28 (1998) 423-4281 measured urinary 8-OHdG of 28 infants (24-40 weeks gestation) and found no gestation or birthweight related differences. This discrepancy seemed to be because of difference in birth weights and sampling period of the subjects.
极低出生体重（VLBW）婴儿在重症监护过程中可能会遭受氧化应激。我们测量了氧化应激的生物标志物8-羟基脱氧鸟苷（8-OHdG），并评估了此类婴儿的氧化应激程度。我们还研究了给予的氧气是否与氧化应激有关。收集了50名日本VLBW婴儿[出生体重：956.3±277.6g，胎龄：28.0±2.6周（平均值±标准差）]在不同出生后天数的尿液样本，并使用酶联免疫吸附测定（ELISA）试剂盒测定8-OHdG水平。16名足月儿作为正常对照。随着采样时体重增加，尿8-OHdG的平均水平下降。8-羟基脱氧鸟苷水平分别为：体重低于1000g的婴儿，29.5±16.4微摩尔/摩尔肌酐（n = 24）；1000 - 1500g的婴儿，23.8±14.9（n = 12）；超过1500g的婴儿，16.1±8.5（n = 14）；以及对照组，10.9±7.2（n = 16）。在体重<1000g组与≥1500g组之间（p = 0.0030）、体重<1000g组与对照组之间（p < 0.0001）以及1000 - 1500g组与对照组之间（p = 0.0108）发现有显著差异。同样，随着采样时孕龄增加，8-OHdG的平均水平下降。8-羟基脱氧鸟苷水平分别为：孕龄后252天（36周）之前的婴儿，27.4±15.5微摩尔/摩尔肌酐（n = 34）；252天后，18.2±12.5（n = 16）。<252天组与对照组之间（p < 0.0001）以及<252天组与≥252天组之间（p = 0.0253）的差异具有统计学意义。在基于环境氧气浓度（21%、22 - 29%和≥30%）划分的三组中，没有显著差异（p = 0.417）。婴儿越早产，氧化应激越强烈，因此，是早产而非给予的氧气导致VLBW婴儿出现氧化应激。Drury等人[《婴儿和儿童尿液中的8-羟基脱氧鸟苷》，《自由基研究》28（1998）423 - 428]测量了28名婴儿（孕龄24 - 40周）的尿8-OHdG，未发现与孕龄或出生体重相关的差异。这种差异似乎是由于研究对象的出生体重和采样期不同所致。
