Opsahl M S, Thorsell L P, Geltinger M E, Iwaszko M A, Blauer K L, Sherins R J
Genetics & IVF Institute, Fairfax, Virginia 22031, USA.
J Assist Reprod Genet. 2002 Mar;19(3):113-7. doi: 10.1023/a:1014728603309.
To determine whether donor oocyte cytoplasm transferred into the oocytes of women < or = 40 years or with diminished ovarian reserve would enhance embryo quality, implantation, or pregnancy rates.
Study subjects included women > or = 40 years (15) or with abnormal FSH levels (3). Healthy volunteers (18) produced oocytes for cryopreservation. Donor oocytes were thawed and cytoplasm from surviving oocytes was injected with a single sperm into the cytoplasm of recipient oocytes. Outcome measures included embryo quality scores, implantation, and pregnancy rates.
Eighteen donors produced 213 oocytes for cryopreservation and 39/171 (22.8%) survived thawing. Eighteen recipients initiated 25 IVF cycles with embryo transfer in 20 cycles after cytoplasmic transfer (CT). Four cycles resulted in three biochemical losses and one aneuploid clinical loss. Embryo quality did not improve with CT compared to pre-CT IVF cycles in six recipients.
CT with cryopreserved donor oocyte cytoplasm did not enhance success in women with advanced reproductive age or low ovarian reserve.
确定将供体卵母细胞胞质转移至年龄≤40岁或卵巢储备功能减退的女性卵母细胞中是否会提高胚胎质量、着床率或妊娠率。
研究对象包括年龄≥40岁的女性（15例）或促卵泡生成素（FSH）水平异常的女性（3例）。健康志愿者（18例）提供用于冷冻保存的卵母细胞。将供体卵母细胞解冻，将存活卵母细胞的胞质与单个精子一起注入受体卵母细胞的胞质中。观察指标包括胚胎质量评分、着床率和妊娠率。
18名供体提供了213个用于冷冻保存的卵母细胞，其中39/171（22.8%）在解冻后存活。18名受体开始了25个体外受精（IVF）周期，在胞质转移（CT）后20个周期进行了胚胎移植。4个周期导致3例生化妊娠失败和1例非整倍体临床妊娠失败。与6名受体CT前的IVF周期相比，CT后胚胎质量并未改善。
将冷冻保存的供体卵母细胞胞质进行CT并不能提高高龄或卵巢储备功能低下女性的成功率。
