Lanzendorf S E, Mayer J F, Toner J, Oehninger S, Saffan D S, Muasher S
The Jones Institute for Reproductive Medicine, Department of Obstetrics and Gynecology, Eastern Virginia Medical School, Norfolk 23507, USA.
Fertil Steril. 1999 Mar;71(3):575-7. doi: 10.1016/s0015-0282(98)00504-4.
To determine if frozen-thawed donor oocytes could be used to provide cytoplasm for transfer into patients' oocytes to improve subsequent embryonic development.
Prospective evaluation of the procedure in consenting IVF patients.
Assisted reproductive technology program.
PATIENT(S): The study was open to consenting IVF patients (of any age) with a history of poor embryo quality or those couples in which the wife's age was > or = 40 years.
INTERVENTION(S): Transfer of donor egg cytoplasm from frozen-thawed oocytes into the oocytes of infertile recipients.
MAIN OUTCOME MEASURE(S): Donor oocyte survival following cryopreservation, fertilization following cytoplasmic transfer into recipient oocytes, embryo quality, and pregnancy outcome.
RESULT(S): Oocytes collected from four donors were cryopreserved and 61% (28/46) survived the thaw procedure. Cytoplasmic transfer was performed on the eggs of four patients, with fertilization occurring in 70.3% (26/37). Twin pregnancy was established in one patient (35 years of age) with a history of poor embryo quality.
CONCLUSION(S): Cryopreserved donor oocytes may provide a source of cytoplasm for transfer into recipient oocytes, eliminating the need for cycle synchronization between donor and infertile patient.
确定冻融后的供体卵母细胞是否可用于提供细胞质，以转移至患者卵母细胞中，从而改善后续胚胎发育情况。
对同意参与的体外受精（IVF）患者的该操作进行前瞻性评估。
辅助生殖技术项目。
该研究对同意参与的有胚胎质量差病史的IVF患者（任何年龄）或妻子年龄≥40岁的夫妇开放。
将冻融卵母细胞的供体卵细胞质转移至不育受体的卵母细胞中。
冷冻保存后供体卵母细胞的存活率、细胞质转移至受体卵母细胞后的受精情况、胚胎质量及妊娠结局。
从4名供体收集的卵母细胞被冷冻保存，61%（28/46）在解冻过程中存活。对4名患者的卵子进行了细胞质转移，70.3%（26/37）发生了受精。1名有胚胎质量差病史的35岁患者成功受孕并怀有双胞胎。
冷冻保存的供体卵母细胞可为转移至受体卵母细胞提供细胞质来源，无需供体与不育患者之间的周期同步。
