Lapidus Alla, Galleron Nathalie, Andersen Jens Tønne, Jørgensen Per Linå, Ehrlich S Dusko, Sorokin Alexei
Génétique Microbienne, INRA, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France.
FEMS Microbiol Lett. 2002 Mar 19;209(1):23-30. doi: 10.1111/j.1574-6968.2002.tb11104.x.
We have established the co-linear regions of Bacillus licheniformis, an industrially important bacterium, and Bacillus subtilis, a model bacterium. In the co-linear regions, revealed by PCR, gene content and order are presumed to be conserved. These regions constitute approximately 60% of the compared chromosomes. Sequencing of the competence genes of B. licheniformis allowed us to validate the approach, and to demonstrate how it can be used for the comparative analysis of complex genetic systems. A new insertion sequence, designated IS3Bli1, was discovered in the competence region of the analyzed B. licheniformis strain.
我们已经确定了工业上重要的地衣芽孢杆菌和模式细菌枯草芽孢杆菌的共线性区域。在通过PCR揭示的共线性区域中，基因含量和顺序被认为是保守的。这些区域约占所比较染色体的60%。地衣芽孢杆菌感受态基因的测序使我们能够验证该方法，并证明其可如何用于复杂遗传系统的比较分析。在分析的地衣芽孢杆菌菌株的感受态区域发现了一个新的插入序列，命名为IS3Bli1。
