通过种间转化实验对枯草芽孢杆菌染色体复制起点附近的几个核糖体蛋白基因进行定位。
Mapping by interspecies transformation experiments of several ribosomal protein genes near the replication origin of Bacillus subtilis chromosome.
作者信息
Osawa S, Tokui A, Saito H
出版信息
Mol Gen Genet. 1978 Aug 17;164(2):113-29. doi: 10.1007/BF00267376.
Bacillus subtilis 168 was transformed with DNAs from B. amyloliquefaciens K or B. licheniformis IAM 11054. These two species show a considerable difference in ribosomal proteins from B. subtilis. Analyses of the transformants indicated that the genes for 16 proteins, S3, S5, S8, S12, S17, S19, BL1, BL5, BL6, BL8, BL14, BL16, BL17, BL22, BL23 and BL25 are located in the cysA-str-spc region on B. subtilis chromosome. The genes for 10 proteins, S4, S6, S13, S16, S20, BL15, BL18, BL20, BL24 and BL28 could not be found in this region in the present experiments.
用解淀粉芽孢杆菌K或地衣芽孢杆菌IAM 11054的DNA转化枯草芽孢杆菌168。这两个物种与枯草芽孢杆菌的核糖体蛋白有很大差异。对转化体的分析表明，16种蛋白质（S3、S5、S8、S12、S17、S19、BL1、BL5、BL6、BL8、BL14、BL16、BL17、BL22、BL23和BL25）的基因位于枯草芽孢杆菌染色体的cysA-str-spc区域。在本实验中，未在该区域发现10种蛋白质（S4、S6、S13、S16、S20、BL15、BL18、BL20、BL24和BL28）的基因。
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