Eckstein B, Yehud S, Shani J, Goldhaber G
J Endocrinol. 1976 Jul;70(1):25-30. doi: 10.1677/joe.0.0700025.
The effect of 5alpha-androstane-3alpha, 17 beta-diol, its 3 beta epimer and oestradiol benzoate on suppression of LH release after ovariectomy was studied in immature rats. At doses of 50 and 100 mug/100 g body weight/day the 3alpha compound suppressed LH release after ovariectomy to the same extent as 0-1 mug oestradiol benzoate/100 g/day. 3alpha-Androstanediol at a dose of 25 mug/100 g/day suppressed LH release only up to day 45 of life, while the same dose of the 3beta epimer had no effect on LH suppression. The effect of 3beta-androstanediol on inducing precocious vaginal opening was found to be mediated by the ovaries, since it was eliminated by ovariectomy. These results confirm our previous findings on the participation of androstanediol in the normal regulation of LH and in the mechanism of onset of puberty in the rat.
在未成熟大鼠中研究了5α-雄甾烷-3α,17β-二醇、其3β差向异构体及苯甲酸雌二醇对卵巢切除术后促黄体生成素（LH）释放抑制的影响。在剂量为50和100μg/100g体重/天时，3α化合物对卵巢切除术后LH释放的抑制程度与0.1μg苯甲酸雌二醇/100g/天相同。剂量为25μg/100g/天的3α-雄甾二醇仅在出生后45天内抑制LH释放，而相同剂量的3β差向异构体对LH抑制无作用。发现3β-雄甾二醇诱导早熟性阴道开口的作用是由卵巢介导的，因为卵巢切除可消除该作用。这些结果证实了我们之前关于雄甾二醇参与大鼠LH正常调节及青春期启动机制的发现。
