Shang Fu, Gong Xin, Egtesadi Shahryar, Meydani Mohsen, Smith Donald, Perrone Gayle, Scott Linda, Blumberg Jeffrey B., Taylor Allen
Jean Mayer USDA Human Nutrition Research, Center on Aging, Tufts University, 02111, Boston, MA, USA
J Nutr Biochem. 2002 May;13(5):307-313. doi: 10.1016/s0955-2863(02)00173-0.
Hyperbaric oxygen therapy is used to treat various clinical conditions, but it also causes oxidative damage. The objectives of this study are to determine if increased vitamin C intake can prevent hyperbaric oxygen-induced damage and to determine interactions among vitamin C, glutathione and vitamin E in response to oxidative stress. The growth rates of unexposed guinea pigs fed 1.25 mg vitamin C/day were indistinguishable from that of guinea pigs fed 50 mg vitamin C/day. In contrast, hyperbaric oxygen exposure resulted in growth retardation in guinea pigs fed 1.25 mg vitamin C/day, but it had little effect on the growth rates of guinea pigs fed 50 mg vitamin C/day. Increased vitamin C intake also prevented hyperbaric oxygen-induced lipid peroxidation in the liver. In guinea pigs not exposed to hyperbaric oxygen, levels of vitamin C in tissues were closely related to vitamin C intake, but tissue levels of glutathione and vitamin E were not related to vitamin C intake. However, interactions between vitamin C and glutathione were observed upon chronic hyperbaric oxygen exposure. Chronic hyperbaric oxygen exposure resulted in >2-fold increases in the levels of glutathione in liver and lung of guinea pigs fed 1.25 mg vitamin C/day. In comparison, the oxidation-induced increases in glutathione were significantly attenuated in guinea pigs fed 50 mg vitamin C/day. These data show that increased intake of vitamin C can prevent or alleviate the hyperbaric oxygen-induced damage. The interactions between vitamin C and glutathione upon hyperbaric oxygen exposure indicate that there is a homeostatic regulation of antioxidant capacity in guinea pig tissues.
高压氧疗法用于治疗各种临床病症，但它也会造成氧化损伤。本研究的目的是确定增加维生素C的摄入量是否能预防高压氧引起的损伤，并确定维生素C、谷胱甘肽和维生素E在应对氧化应激时的相互作用。每天摄入1.25毫克维生素C的未接受高压氧暴露的豚鼠的生长速率与每天摄入50毫克维生素C的豚鼠的生长速率没有区别。相比之下，高压氧暴露导致每天摄入1.25毫克维生素C的豚鼠生长迟缓，但对每天摄入50毫克维生素C的豚鼠的生长速率影响很小。增加维生素C的摄入量还可预防高压氧引起的肝脏脂质过氧化。在未接受高压氧暴露的豚鼠中，组织中的维生素C水平与维生素C摄入量密切相关，但谷胱甘肽和维生素E的组织水平与维生素C摄入量无关。然而，在慢性高压氧暴露时观察到了维生素C和谷胱甘肽之间的相互作用。慢性高压氧暴露导致每天摄入1.25毫克维生素C的豚鼠肝脏和肺中谷胱甘肽水平增加两倍以上。相比之下，每天摄入50毫克维生素C的豚鼠中，氧化诱导的谷胱甘肽增加明显减弱。这些数据表明，增加维生素C的摄入量可以预防或减轻高压氧引起的损伤。高压氧暴露时维生素C和谷胱甘肽之间的相互作用表明，豚鼠组织中抗氧化能力存在稳态调节。
